Product Name
Hepatocyte Nuclear Factor 1 Beta (HNF1b), ELISA Kit
Full Product Name
Rat Hepatocyte Nuclear Factor 1 Beta ELISA Kit (HNF1b)
Product Gene Name
HNF1b elisa kit
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Request Current Manual
3D Structure
ModBase 3D Structure for P35680
Assay Type
Sandwich ELISA
Samples
Cell Culture Supernatant, Serum, Plasma (EDTA, Citrate, Heparin)
Detection Range
0.156-10 ng/mL
Preparation and Storage
Store at 4 degree C.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of HNF1b elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for HNF1b purchase
MBS9139248 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Hepatocyte Nuclear Factor 1 Beta (HNF1b) ELISA Kit target analytes in
biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing HNF1b. The ELISA analytical biochemical technique of the MBS9139248 kit is based on HNF1b antibody-HNF1b antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HNF1b antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HNF1b. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
HNF1b elisa kit
Product Overview: The ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of samples in cell culture supernatant, serum, plasma (EDTA, citrate, heparin).
Intended Use: This Rat Hepatocyte Nuclear Factor 1 Beta (HNF1b) ELISA kit is intended for laboratory research use only and not for use in diagnostic or therapeutic procedures. The stop solution changes color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of Rat Hepatocyte Nuclear Factor 1 Beta (HNF1b) in the sample, this Rat Hepatocyte Nuclear Factor 1 Beta (HNF1b) ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of optical density versus Rat Hepatocyte Nuclear Factor 1 Beta (HNF1b) concentration. The concentration of the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI/Uniprot data below describe general gene information for HNF1b. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000449.1
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NCBI GenBank Nucleotide #
NM_000458.4
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UniProt Primary Accession #
P35680
[Other Products]
UniProt Secondary Accession #
B4DKM3; E0YMJ9[Other Products]
UniProt Related Accession #
P35680[Other Products]
Molecular Weight
58,379 Da
NCBI Official Full Name
hepatocyte nuclear factor 1-beta isoform 1
NCBI Official Synonym Full Names
HNF1 homeobox B
NCBI Official Symbol
HNF1B??[Similar Products]
NCBI Official Synonym Symbols
FJHN; HNF2; LFB3; TCF2; HPC11; LF-B3; MODY5; TCF-2; VHNF1; HNF-1B; HNF1beta; HNF-1-beta
??[Similar Products]
NCBI Protein Information
hepatocyte nuclear factor 1-beta
UniProt Protein Name
Hepatocyte nuclear factor 1-beta
UniProt Synonym Protein Names
Homeoprotein LFB3; Transcription factor 2; TCF-2; Variant hepatic nuclear factor 1; vHNF1
Protein Family
Hepatocyte nuclear factor
UniProt Gene Name
HNF1B??[Similar Products]
UniProt Synonym Gene Names
TCF2; HNF-1-beta; HNF-1B; TCF-2; vHNF1??[Similar Products]
NCBI Summary for HNF1b
This gene encodes a member of the homeodomain-containing superfamily of transcription factors. The protein binds to DNA as either a homodimer, or a heterodimer with the related protein hepatocyte nuclear factor 1-alpha. The gene has been shown to function in nephron development, and regulates development of the embryonic pancreas. Mutations in this gene result in renal cysts and diabetes syndrome and noninsulin-dependent diabetes mellitus, and expression of this gene is altered in some types of cancer. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009]
UniProt Comments for HNF1b
Transcription factor, probably binds to the inverted palindrome 5'-GTTAATNATTAAC-3'.
Research Articles on HNF1b
1. HNF1B p.Arg527Gln nonsense mutation mutation is associated with atypical early onset diabetes and diabetic nephropathy.
Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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