Product Name
MYO7A, siRNA
Full Product Name
MYO7A siRNA (Mouse)
Product Synonym Names
MYO7; Unconventional myosin-VIIa
Product Gene Name
MYO7A sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P97479
Specificity
MYO7A siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse MYO7A gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of MYO7A sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
MYO7A sirna
siRNA to inhibit MYO7A expression using RNA interference
Applications Tested/Suitable for MYO7A sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for MYO7A. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001243010.1
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NCBI GenBank Nucleotide #
NM_001256081.1
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UniProt Primary Accession #
P97479
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UniProt Secondary Accession #
Q5MJ57; E9QLP7[Other Products]
UniProt Related Accession #
P97479[Other Products]
Molecular Weight
251,097 Da
NCBI Official Full Name
unconventional myosin-VIIa isoform 1
NCBI Official Synonym Full Names
myosin VIIA
NCBI Official Symbol
Myo7a??[Similar Products]
NCBI Official Synonym Symbols
Hdb; sh1; Myo7; sh-1; USH1B; polka; nmf371
??[Similar Products]
NCBI Protein Information
unconventional myosin-VIIa
UniProt Protein Name
Unconventional myosin-VIIa
Protein Family
Unconventional myosin
UniProt Gene Name
Myo7a??[Similar Products]
UniProt Synonym Gene Names
Myo7??[Similar Products]
UniProt Entry Name
MYO7A_MOUSE
UniProt Comments for MYO7A
MYO7A: an actin-based motor molecule with ATPase activity and a calcium sensitive calmodulin binding subunit. May play a role in trafficking of ribbon- synaptic vesicle complexes and renewal of outer photoreceptor disks. Involved in hair-cell vesicle trafficking of aminoglycosides, which are known to induce ototoxicity. Seven alternatively spliced isoforms have been described.
Protein type: Motility/polarity/chemotaxis; Motor
Cellular Component: photoreceptor inner segment; microvillus; lysosomal membrane; cell; cytosol; stereocilium; cytoskeleton; photoreceptor outer segment; cytoplasm; apical plasma membrane; melanosome; synapse; intracellular; myosin complex; photoreceptor connecting cilium
Molecular Function: protein domain specific binding; protein homodimerization activity; motor activity; nucleotide binding; protein N-terminus binding; actin-dependent ATPase activity; actin binding; ADP binding; calmodulin binding; microfilament motor activity; actin filament binding; protein binding; spectrin binding; protein complex binding; ATP binding
Biological Process: inner ear morphogenesis; mechanoreceptor differentiation; actin filament-based movement; auditory receptor cell differentiation; phagocytosis; phagolysosome formation; intracellular protein transport; pigment granule transport; sensory perception of sound; inner ear receptor cell differentiation; cell projection organization and biogenesis; visual perception; lysosome organization and biogenesis; transport; sensory perception of light stimulus; sensory perception; auditory receptor cell stereocilium organization and biogenesis; equilibrioception; inner ear development; post-embryonic organ morphogenesis; pigment granule localization
Research Articles on MYO7A
1. Myo7a interacts with integrin beta5 and selectively promotes integrin alphavbeta5-mediated cell migration
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
