Product Name
3-phosphoinositide-dependent protein kinase 1 (Pdpk1), Recombinant Protein
Full Product Name
Recombinant Rat 3-phosphoinositide-dependent protein kinase 1 (Pdpk1)
Product Gene Name
Pdpk1 recombinant protein
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence Positions
1-559aa, Full length protein
Sequence
MARTTSQLYD AVPIQSSVVL CSCPSPSMVR SQTEPSSSPG IPSGVSRQGS TMDGTTAEAR PSTNPLQQHP AQLPPQPRKK RPEDFKFGKI LGEGSFSTVV LARELATSRE YAIKILEKRH IIKENKVPYV TRERDVMSRL DHPFFVKLYF TFQDDEKLYF GLSYAKNGEL LKYIRKIGSF DETCTRFYTA EIVSALEYLH GKGIIHRDLK PENILLNEDM HIQITDFGTA KVLSPDSKQA RANSFVGTAQ YVSPELLTEK SACKSSDLWA LGCIIYQLVA GLPPFRAGNE YLIFQKIIKL EYDFPEKFFP KARDLVEKLL VLDATKRLGC EEMEGYGPLK AHPFFESITW ENLHQQTPPK LTAYLPAMSE DDEDCYGNYD NLLSQFGCMQ VSSSSSSHSL SAVDASLPQR SGSNIEQYIH DLDTNSFELD LQFSEDEKRL LLEKQAGGNP WHQFVENNLI LKMGPVDKRK GLFARRRQLL LTEGPHLYYV DPVNKVLKGE IPWSQELRPE AKNFKTFFVH TPNRTYYLMD PSGNAHKWCR KIQEVWRQQY QSSPDAAVQ
3D Structure
ModBase 3D Structure for O55173
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Purity/Purification
>85% (SDS-PAGE) (lot specific)
Form/Format
Liquid containing glycerol
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
Storage Buffer
Tris-based buffer, 50% glycerol
Preparation and Storage
Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Other Notes
Small volumes of Pdpk1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
NCBI/Uniprot data below describe general gene information for Pdpk1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_112343.1
[Other Products]
NCBI GenBank Nucleotide #
NM_031081.1
[Other Products]
UniProt Primary Accession #
O55173
[Other Products]
UniProt Secondary Accession #
G3V9W3[Other Products]
UniProt Related Accession #
O55173[Other Products]
Molecular Weight
63,593 Da
NCBI Official Full Name
3-phosphoinositide-dependent protein kinase 1
NCBI Official Synonym Full Names
3-phosphoinositide dependent protein kinase-1
NCBI Official Symbol
Pdpk1??[Similar Products]
NCBI Protein Information
3-phosphoinositide-dependent protein kinase 1
UniProt Protein Name
3-phosphoinositide-dependent protein kinase 1
UniProt Synonym Protein Names
Protein kinase B kinase; PkB kinase
Protein Family
3-phosphoinositide-dependent protein kinase
UniProt Gene Name
Pdpk1??[Similar Products]
UniProt Synonym Gene Names
Pdk1; PkB kinase??[Similar Products]
NCBI Summary for Pdpk1
may catalyze the phosphorylation and activation of protein kinase B [RGD, Feb 2006]
UniProt Comments for Pdpk1
Serine/threonine kinase which acts as a master kinase, phosphorylating and activating a subgroup of the AGC family of protein kinases. Its targets include: protein kinase B (PKB/AKT1, PKB/AKT2, PKB/AKT3), p70 ribosomal protein S6 kinase (RPS6KB1), p90 ribosomal protein S6 kinase (RPS6KA1, RPS6KA2 and RPS6KA3), cyclic AMP-dependent protein kinase (PRKACA), protein kinase C (PRKCD and PRKCZ), serum and glucocorticoid-inducible kinase (SGK1, SGK2 and SGK3), p21-activated kinase-1 (PAK1), protein kinase PKN (PKN1 and PKN2). Plays a central role in the transduction of signals from insulin by providing the activating phosphorylation to PKB/AKT1, thus propagating the signal to downstream targets controlling cell proliferation and survival, as well as glucose and amino acid uptake and storage. Negatively regulates the TGF-beta-induced signaling by: modulating the association of SMAD3 and SMAD7 with TGF-beta receptor, phosphorylating SMAD2, SMAD3, SMAD4 and SMAD7, preventing the nuclear translocation of SMAD3 and SMAD4 and the translocation of SMAD7 from the nucleus to the cytoplasm in response to TGF-beta. Activates PPARG transcriptional activity and promotes adipocyte differentiation. Activates the NF-kappa-B pathway via phosphorylation of IKKB. The tyrosine phosphorylated form is crucial for the regulation of focal adhesions by angiotensin II. Controls proliferation, survival, and growth of developing pancreatic cells. Participates in the regulation of Ca2+ entry and Ca2+-activated K+ channels of mast cells. Essential for the motility of vascular endothelial cells (ECs) and is involved in the regulation of their chemotaxis. Plays a critical role in cardiac homeostasis by serving as a dual effector for cell survival and beta-adrenergic response. Plays an important role during thymocyte development by regulating the expression of key nutrient receptors on the surface of pre-T cells and mediating Notch-induced cell growth and proliferative responses. Provides negative feedback inhibition to toll-like receptor-mediated NF-kappa-B activation in macrophages ().
Research Articles on Pdpk1
1. PI3K, PDK1, and PKCzeta have roles independent of insulin, in inducing glucose uptake
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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