3-hydroxy-3-methylglutaryl-coenzyme A reductase; HMG-CoA reductase; Hmgcr; 1.1.1.34

For Research Use Only. Not for use in diagnostic procedures.

For long term storage, please store the entire kit at -20 degree C.

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Small volumes of Hmgcr elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS2880097 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 3-hydroxy-3-methylglutaryl-coenzyme A reductase (Hmgcr) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Hmgcr. The ELISA analytical biochemical technique of the MBS2880097 kit is based on Hmgcr antibody-Hmgcr antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Hmgcr antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Hmgcr. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

97,040 Da

3-hydroxy-3-methylglutaryl-Coenzyme A reductase

3-hydroxy-3-methylglutaryl-coenzyme A reductase

3-hydroxy-3-methylglutaryl-coenzyme A reductase

HMG-CoA reductase??[Similar Products]

HMDH_MOUSE

HMGCR: the rate-limiting enzyme for cholesterol synthesis. Regulated via a negative feedback mechanism mediated by sterols and non-sterol metabolites derived from mevalonate, the product of the reaction catalyzed by this reductase. Normally in mammalian cells this enzyme is suppressed by cholesterol derived from the internalization and degradation of low density lipoprotein (LDL) via the LDL receptor. Competitive inhibitors of the reductase induce the expression of LDL receptors in the liver, which in turn increases the catabolism of plasma LDL and lowers the plasma concentration of cholesterol, an important determinant of atherosclerosis.

Protein type: EC 1.1.1.34; Oxidoreductase; Endoplasmic reticulum; Membrane protein, integral; Secondary Metabolites Metabolism - terpenoid backbone biosynthesis; Motility/polarity/chemotaxis; Membrane protein, multi-pass

Cellular Component: endoplasmic reticulum; endoplasmic reticulum membrane; integral to membrane; intracellular membrane-bound organelle; membrane; peroxisomal membrane

Molecular Function: coenzyme binding; hydroxymethylglutaryl-CoA reductase (NADPH) activity; hydroxymethylglutaryl-CoA reductase activity; NADP binding; oxidoreductase activity; oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor; protein homodimerization activity; protein phosphatase 2A binding

Biological Process: cholesterol biosynthetic process; cholesterol metabolic process; coenzyme A metabolic process; embryonic development; isoprenoid biosynthetic process; lipid metabolic process; negative regulation of apoptosis; negative regulation of MAP kinase activity; negative regulation of vasodilation; positive regulation of cell proliferation; positive regulation of skeletal muscle development; positive regulation of smooth muscle cell proliferation; positive regulation of stress-activated MAPK cascade; protein tetramerization; steroid biosynthetic process; steroid metabolic process; sterol biosynthetic process; ubiquinone metabolic process; visual learning

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