DNT; DNT1; P5N2; PN-I; PN-II; UMPH2; cdN; 5'Nucleotidase,Deoxy(Pyrimidine)Cytosolic Type C; Uridine 5-Prime Monophosphate Hydrolase 2; 5'(3')-deoxyribonucleotidase, cytosolic

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

IgG

Rabbit

The antibody is a rabbit polyclonal antibody raised against NT5C. It has been selected for its ability to recognize NT5C in immunohistochemical staining and western blotting.

Affinity Chromatography

Supplied as solution form in PBS, pH7.4, Containing 0.02% NaN3, 50% glycerol.

200ug/ml (lot specific)

Store at 4 degree C for frequent use. Stored at -20 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.

Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customera??s specifications, please inquire.

Small volumes of anti-NT5C antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)

Western blotting: 1:50-400
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-1:5000

Western Blot: Sample: Mouse Stomach lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse NT5C Antibody Second Ab: 0.2ug/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody

Western Blot: Sample: Recombinant protein.

DAB staining on fromalin fixed paraffin- embedded heart tissue)

DAB staining on IHC-P; Samples: Mouse Skeletal muscle Tissue)

23,076 Da

5',3'-nucleotidase, cytosolic

5'(3')-deoxyribonucleotidase, cytosolic type

5'(3')-deoxyribonucleotidase, cytosolic type

Dnt1; dNT-1??[Similar Products]

NT5C: Dephosphorylates the 5' and 2'(3')-phosphates of deoxyribonucleotides, with a preference for dUMP and dTMP, intermediate activity towards dGMP, and low activity towards dCMP and dAMP. Belongs to the 5'(3')-deoxyribonucleotidase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; EC 3.1.3.-; Hydrolase; Nucleotide Metabolism - purine; Nucleotide Metabolism - pyrimidine

Chromosomal Location of Human Ortholog: 11|11 E2

Cellular Component: cytoplasm; cytosol; mitochondrion; nucleus

Molecular Function: 5'-nucleotidase activity; hydrolase activity; metal ion binding; nucleotidase activity; nucleotide binding; phosphoric monoester hydrolase activity; pyrimidine nucleotide binding

Biological Process: deoxyribonucleotide catabolic process; dephosphorylation; nucleotide metabolic process; pyrimidine deoxyribonucleotide catabolic process

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