Human Astacin-like metalloendopeptidase (ASTL) ELISA kit; MGC129843; astacin-like metalloendopeptidase; astacin-like metalloendopeptidase (M12 family) ; ovastacin; astacin-like metallo-endopeptidase (M12 family)

For Research Use Only. Not for use in diagnostic procedures.

Store all reagents at 2-8 degree C

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Small volumes of ASTL elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS9320857 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the astacin-like metallo-endopeptidase (M12 family) (ASTL) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ASTL. The ELISA analytical biochemical technique of the MBS9320857 kit is based on ASTL antibody-ASTL antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ASTL antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ASTL. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

45,936 Da

astacin-like metallo-endopeptidase (M12 family)

astacin-like metalloendopeptidase; ovastacin; oocyte astacin; sperm acrosomal SLLP1 binding; astacin-like metalloendopeptidase (M12 family)

Astacin-like metalloendopeptidase

ASTL_HUMAN

ASTL: Oocyte-specific oolemmal receptor involved in sperm and egg adhesion and fertilization. Plays a role in the polyspermy inhibition. Probably acts as a protease for the post-fertilization cleavage of ZP2. Cleaves the sperm-binding ZP2 at the surface of the zona pellucida after fertilization and cortical granule exocytosis, rendering the zona pellucida unable to support further sperm binding. Belongs to the peptidase M12A family.

Protein type: EC 3.4.-.-; Protease

Chromosomal Location of Human Ortholog: 2q11.1

Cellular Component: transport vesicle; cytoplasm; plasma membrane

Molecular Function: peptidase activity; zinc ion binding; metalloendopeptidase activity

Biological Process: fertilization; proteolysis; cell adhesion

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