DNT; DNT1; P5N2; PN-I; PN-II; UMPH2; cdN; 5'Nucleotidase,Deoxy(Pyrimidine)Cytosolic Type C; Uridine 5-Prime Monophosphate Hydrolase 2; 5'(3')-deoxyribonucleotidase, cytosolic

For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of 5',3'-Nucleotidase, Cytosolic (NT5C).
No significant cross-reactivity or interference between 5',3'-Nucleotidase, Cytosolic (NT5C) and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.

Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customera??s specifications, please inquire.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of NT5C elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS2705304 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 5',3'-Nucleotidase, Cytosolic (NT5C) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing NT5C. The ELISA analytical biochemical technique of the MBS2705304 kit is based on NT5C antibody-NT5C antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NT5C antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NT5C. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 5',3'-Nucleotidase, Cytosolic (NT5C). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to 5',3'-Nucleotidase, Cytosolic (NT5C). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain 5',3'-Nucleotidase, Cytosolic (NT5C), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 5',3'-Nucleotidase, Cytosolic (NT5C) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

13,299 Da

5', 3'-nucleotidase, cytosolic

5'(3')-deoxyribonucleotidase, cytosolic type

5'(3')-deoxyribonucleotidase, cytosolic type

DNT1; UMPH2; dNT-1??[Similar Products]

This gene encodes a nucleotidase that catalyzes the dephosphorylation of the 5' deoxyribonucleotides (dNTP) and 2'(3')-dNTP and ribonucleotides, but not 5' ribonucleotides. Of the different forms of nucleotidases characterized, this enzyme is unique in its preference for 5'-dNTP. It may be one of the enzymes involved in regulating the size of dNTP pools in cells. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Nov 2011]

NT5C: Dephosphorylates the 5' and 2'(3')-phosphates of deoxyribonucleotides, with a preference for dUMP and dTMP, intermediate activity towards dGMP, and low activity towards dCMP and dAMP. Belongs to the 5'(3')-deoxyribonucleotidase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; EC 3.1.3.-; Hydrolase; Nucleotide Metabolism - purine; Nucleotide Metabolism - pyrimidine

Chromosomal Location of Human Ortholog: 17q25.1

Cellular Component: cytoplasm; cytosol; mitochondrion; nucleus

Molecular Function: 5'-nucleotidase activity; metal ion binding; nucleotidase activity; pyrimidine nucleotide binding

Biological Process: dephosphorylation; purine nucleotide catabolic process; pyrimidine deoxyribonucleotide catabolic process; pyrimidine nucleoside catabolic process

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