For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit

Affinity Purified

Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

For short term use, store at 2-8 degree C up to 1 week. For long term storage, store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Small volumes of anti-CYP21A2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

This is a rabbit polyclonal antibody against CYP21A2. It was validated on Western Blot

Target Description: This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and hydroxylates steroids at the 21 position. Its activity is required for the synthesis of steroid hormones including cortisol and aldosterone. Mutations in this gene cause congenital adrenal hyperplasia. A related pseudogene is located near this gene; gene conversion events involving the functional gene and the pseudogene are thought to account for many cases of steroid 21-hydroxylase deficiency. Two transcript variants encoding different isoforms have been found for this gene.

Polyclonal; Drugs and Drug Metabolism; Mitochondria; Disease Related;

Immunohistochemistry (IHC), Western Blot (WB)

Sample Type: Monkey adrenal gland
Sample Type :
Monkey adrenal gland
Primary Antibody Dilution :
1:25
Secondary Antibody:
Anti-rabbit-HRP
Secondary Antibody Dilution:
1:1000
Color/Signal Descriptions:
Brown: CYP21A2 Blue: Nucleus
Gene Name:
CYP21A2
Submitted by:
Jonathan Bertin, Endoceutics Inc.

Sample Type: Monkey vagina
Sample Type :
Monkey vagina
Primary Antibody Dilution :
1:25
Secondary Antibody:
Anti-rabbit-HRP
Secondary Antibody Dilution:
1:1000
Color/Signal Descriptions:
Brown: CYP21A2 Blue: Nucleus
Gene Name:
CYP21A2
Submitted by:
Jonathan Bertin, Endoceutics Inc.

WB Suggested Anti-CYP21A2 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:62500
Positive Control: Human Muscle

56kDa

cytochrome P450 family 21 subfamily A member 2

steroid 21-hydroxylase

Cytochrome P450 21-hydroxylase

CYP21A2??[Similar Products]

Q16874_HUMAN

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and hydroxylates steroids at the 21 position. Its activity is required for the synthesis of steroid hormones including cortisol and aldosterone. Mutations in this gene cause congenital adrenal hyperplasia. A related pseudogene is located near this gene; gene conversion events involving the functional gene and the pseudogene are thought to account for many cases of steroid 21-hydroxylase deficiency. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

CYP21A2: Specifically catalyzes the 21-hydroxylation of steroids. Required for the adrenal synthesis of mineralocorticoids and glucocorticoids. Defects in CYP21A2 are the cause of adrenal hyperplasia type 3 (AH3). AH3 is a form of congenital adrenal hyperplasia, a common recessive disease due to defective synthesis of cortisol. Congenital adrenal hyperplasia is characterized by androgen excess leading to ambiguous genitalia in affected females, rapid somatic growth during childhood in both sexes with premature closure of the epiphyses and short ***** stature. Four clinical types: 'salt wasting' (SW, the most severe type), 'simple virilizing' (SV, less severely affected patients), with normal aldosterone biosynthesis, 'non-classic form' or late onset (NC or LOAH), and 'cryptic' (asymptomatic). Belongs to the cytochrome P450 family.

Protein type: Lipid Metabolism - C21-steroid hormone; EC 1.14.99.10; Oxidoreductase

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: endoplasmic reticulum membrane

Molecular Function: steroid 21-monooxygenase activity; iron ion binding; heme binding; steroid hydroxylase activity; steroid binding

Biological Process: steroid metabolic process; xenobiotic metabolic process; mineralocorticoid biosynthetic process; glucocorticoid biosynthetic process; sterol metabolic process

Disease: Adrenal Hyperplasia, Congenital, Due To 21-hydroxylase Deficiency

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