DKFZp547C074; DMP4; RNS; dentin matrix protein 4

For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of Mouse FAM20C. No significant cross-reactivity or interference between Mouse FAM20C and analogues was observed.

Store at 2-8 degree C.

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Small volumes of FAM20C elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS284875 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Dentin matrix protein 4 (FAM20C) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing FAM20C. The ELISA analytical biochemical technique of the MBS284875 kit is based on FAM20C antibody-FAM20C antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect FAM20C antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, FAM20C. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate FAM20C in samples. An antibody specific for FAM20C has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyFAM20C present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FAM20C is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FAM20C bound in the initial step. The color development is stopped and the intensity of the color is measured.

65,766 Da

family with sequence similarity 20, member C

extracellular serine/threonine protein kinase FAM20C

Extracellular serine/threonine protein kinase FAM20C

GEF-CK??[Similar Products]

FAM20C: an atypical serine protein kinase that localizes within the Golgi apparatus and is secreted. Distantly related to the Drosophila Golgi-localized kinase Four-jointed. Phosphorylates secretory proteins including the caseins and members of the small integrin-binding ligand, N-linked glycoprotein (SIBLING) protein family, which modulate biomineralization. Colocalizes with the Golgi resident protein GM130. Preferentially phosphorylates its targets within the S-x-E/pS motif. Requires Mn2+ as an alternative to Mg2+ as the activating cation. Defects in FAM20C are the cause of Raine syndrome (RNS). RNS is an autosomal recessive osteosclerotic bone dysplasia with neonatal lethal outcome. Clinical features include generalized osteosclerosis, craniofacial dysplasia and microcephaly. Two isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 5|5 G2

Cellular Component: cytoplasm; extracellular region; extracellular space; Golgi apparatus

Molecular Function: ATP binding; calcium ion binding; kinase activity; manganese ion binding; metal ion binding; nucleotide binding; protein serine/threonine kinase activity; transferase activity

Biological Process: ATP metabolic process; biomineral formation; phosphorylation; positive regulation of bone mineralization; positive regulation of osteoblast differentiation; protein amino acid phosphorylation; regulation of fibroblast growth factor receptor signaling pathway; regulation of phosphorus metabolic process; skeletal system development

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