AXA1; Aprataxin; Forkhead-associated domain histidine triad-like protein; FHA-HIT

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit

Recognizes endogenous levels of APTX protein.

The antibody was purified by immunogen affinity chromatography.

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.

Small volumes of anti-APTX antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Rabbit polyclonal antibody to APTX

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

WB: 1/500 - 1/2000; IHC: 1/50 - 1/200; IF/IC: 1/50 - 1/200

Western blot analysis of APTX expression in A549 (A), K562 (B), Raji (C) whole cell lysates.

Immunohistochemical analysis of APTX staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of APTX staining in K562 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

21,389 Da

aprataxin

aprataxin

Aprataxin

AXA1; FHA-HIT??[Similar Products]

APTX_HUMAN

This gene encodes a member of the histidine triad (HIT) superfamily. The encoded protein may play a role in single-stranded DNA repair through its nucleotide-binding activity and its diadenosine polyphosphate hydrolase activity. Mutations in this gene have been associated with ataxia-ocular apraxia. Alternatively spliced transcript variants have been identified for this gene.[provided by RefSeq, Aug 2010]

APTX: DNA-binding protein involved in single-strand DNA break repair, double-strand DNA break repair and base excision repair. Resolves abortive DNA ligation intermediates formed either at base excision sites, or when DNA ligases attempt to repair non- ligatable breaks induced by reactive oxygen species. Catalyzes the release of adenylate groups covalently linked to 5'-phosphate termini, resulting in the production of 5'-phosphate termini that can be efficiently rejoined. Also able to hydrolyze adenosine 5'- monophosphoramidate (AMP-NH(2)) and diadenosine tetraphosphate (AppppA), but with lower catalytic activity. Defects in APTX are the cause of ataxia-oculomotor apraxia syndrome (AOA). AOA is an autosomal recessive syndrome characterized by early-onset cerebellar ataxia, oculomotor apraxia, early areflexia and late peripheral neuropathy. 13 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding; C2H2-type zinc finger protein; DNA repair, damage; Nucleolus; EC 3.-.-.-; Phosphatase (non-protein)

Chromosomal Location of Human Ortholog: 9p13.3

Cellular Component: chromatin; nuclear chromatin; nucleolus; nucleoplasm; nucleus

Molecular Function: chromatin binding; damaged DNA binding; DNA 5'-adenosine monophosphate hydrolase activity; double-stranded DNA binding; double-stranded RNA binding; phosphoglycolate phosphatase activity; phosphoprotein binding; protein binding; protein N-terminus binding

Biological Process: regulation of protein stability; response to DNA damage stimulus; response to hydrogen peroxide; single strand break repair

Disease: Ataxia, Early-onset, With Oculomotor Apraxia And Hypoalbuminemia

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