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Mediterranean Fever (MEFV), ELISA Kit

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產(chǎn)品名稱: Mediterranean Fever (MEFV), ELISA Kit
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Mediterranean Fever (MEFV), ELISA Kit


Mediterranean Fever (MEFV), ELISA Kit  的詳細介紹
Product Name

Mediterranean Fever (MEFV), ELISA Kit

Full Product Name

Human Mediterranean Fever (MEFV) ELISA Kit

Product Synonym Names
MEF; FMF; TRIM20; Pyrin; Marenostrin
Product Gene Name

MEFV elisa kit

[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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Sample Manual Insert
Download Sample PDF Manual View Sample PDF Manual
Request for Current Manual Insert
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OMIM
AF018080 mRNA
Species Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Mediterranean Fever (MEFV).
No significant cross-reactivity or interference between Mediterranean Fever (MEFV) and analogues was observed.
Assay Type
Double-antibody Sandwich
Samples
Serum, Plasma and Other Biological Fluids
Detection Range
31.2-2000pg/mL
Sensitivity
< 12.4pg/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Mediterranean Fever (MEFV) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Mediterranean Fever (MEFV) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customera??s specifications, please inquire.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of MEFV elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for MEFV purchase
MBS2704577 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Mediterranean Fever (MEFV) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MEFV. The ELISA analytical biochemical technique of the MBS2704577 kit is based on MEFV antibody-MEFV antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MEFV antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MEFV. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for
MEFV elisa kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mediterranean Fever (MEFV). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mediterranean Fever (MEFV). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mediterranean Fever (MEFV), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mediterranean Fever (MEFV) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of MEFV elisa kit
MEFV elisa kit Typical Testing Data/Standard Curve (for reference only) image
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NCBI/Uniprot data below describe general gene information for MEFV. It may not necessarily be applicable to this product.
NCBI GI #
75517693
NCBI GeneID
4210
NCBI Accession #
AAI01538.1 [Other Products]
UniProt Secondary Accession #
Q3MJ84; Q96PN4; Q96PN5; D3DUC0; F5H0Q3[Other Products]
UniProt Related Accession #
O15553[Other Products]
Molecular Weight
50,837 Da
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NCBI Official Full Name
Mediterranean fever
NCBI Official Synonym Full Names
MEFV, pyrin innate immunity regulator
NCBI Official Symbol
MEFV??[Similar Products]
NCBI Official Synonym Symbols
FMF; MEF; TRIM20
??[Similar Products]
NCBI Protein Information
pyrin
UniProt Protein Name
Pyrin
UniProt Synonym Protein Names
Marenostrin
Protein Family
Pyrin
UniProt Gene Name
MEFV??[Similar Products]
UniProt Synonym Gene Names
??[Similar Products]
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NCBI Summary for MEFV
This gene encodes a protein, also known as pyrin or marenostrin, that is an important modulator of innate immunity. Mutations in this gene are associated with Mediterranean fever, a hereditary periodic fever syndrome. [provided by RefSeq, Jul 2008]
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UniProt Comments for MEFV
pyrin: Probably controls the inflammatory response in myelomonocytic cells at the level of the cytoskeleton organization. Defects in MEFV are the cause of familial Mediterranean fever autosomal recessive (ARFMF). ARFMF is an inherited disorder characterized by recurrent episodic fever, serosal inflammation and pain in the abdomen, chest or joints. ARFMF is frequently complicated by amyloidosis, which leads to renal failure and can be prophylactically treated with colchicine. ARFMF primarily affects ancestral ethnic groups living around the Mediterranean basin: North African Jews, Armenians, Arabs and Turks. The disease is also distributed in other populations including Greeks, Cypriots, Italians and Spanish, although at a lower prevalence. Defects in MEFV are the cause of familial Mediterranean fever autosomal dominant (ADFMF). ADFMF is characterized by periodic fever, serosal inflammation and pain in the abdomen, chest or joints as seen also in the autosomal recessive form of the disease. It is associated with renal amyloidosis and characterized by colchicine unresponsiveness. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Actin-binding

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: autophagic vacuole; cytoplasm; cytoplasmic vesicle; cytosol; lamellipodium; microtubule; microtubule associated complex; nucleus; ruffle

Molecular Function: actin binding; identical protein binding; protein binding; zinc ion binding

Biological Process: inflammatory response; negative regulation of cytokine production involved in inflammatory response; negative regulation of inflammatory response; negative regulation of interleukin-1 beta production; negative regulation of interleukin-12 production; negative regulation of macrophage inflammatory protein 1 alpha production; negative regulation of NLRP3 inflammasome complex assembly; positive regulation of autophagy; positive regulation of cysteine-type endopeptidase activity

Disease: Familial Mediterranean Fever; Familial Mediterranean Fever, Autosomal Dominant
Research Articles on MEFV
1. Contrary to the NLRP3 mutations described in cryopyrin-associated periodic syndrome, FMF-associated MEFV mutations do not lead to a constitutive activation of Pyrin. Rather, FMF-associated mutations are hypermorphic mutations that specifically decrease the activation threshold of the Pyrin inflammasome without affecting other canonical inflammasomes.
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Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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