Full Product Name
MEFV siRNA (Mouse)
Product Synonym Names
Pyrin; Marenostrin
Product Gene Name
MEFV sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q9JJ26
Specificity
MEFV siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse MEFV gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of MEFV sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
MEFV sirna
siRNA to inhibit MEFV expression using RNA interference
Applications Tested/Suitable for MEFV sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for MEFV. It may not necessarily be applicable to this product.
NCBI Accession #
NP_062326.2
[Other Products]
NCBI GenBank Nucleotide #
NM_019453.2
[Other Products]
UniProt Primary Accession #
Q9JJ26
[Other Products]
UniProt Secondary Accession #
E9QN30[Other Products]
UniProt Related Accession #
Q9JJ26[Other Products]
Molecular Weight
86,409 Da
NCBI Official Full Name
pyrin isoform 3
NCBI Official Synonym Full Names
Mediterranean fever
NCBI Official Symbol
Mefv??[Similar Products]
NCBI Official Synonym Symbols
FMF; pyrin; TRIM20
??[Similar Products]
NCBI Protein Information
pyrin
UniProt Protein Name
Pyrin
UniProt Synonym Protein Names
Marenostrin
UniProt Gene Name
Mefv??[Similar Products]
UniProt Entry Name
MEFV_MOUSE
UniProt Comments for MEFV
pyrin: Probably controls the inflammatory response in myelomonocytic cells at the level of the cytoskeleton organization. Defects in MEFV are the cause of familial Mediterranean fever autosomal recessive (ARFMF). ARFMF is an inherited disorder characterized by recurrent episodic fever, serosal inflammation and pain in the abdomen, chest or joints. ARFMF is frequently complicated by amyloidosis, which leads to renal failure and can be prophylactically treated with colchicine. ARFMF primarily affects ancestral ethnic groups living around the Mediterranean basin: North African Jews, Armenians, Arabs and Turks. The disease is also distributed in other populations including Greeks, Cypriots, Italians and Spanish, although at a lower prevalence. Defects in MEFV are the cause of familial Mediterranean fever autosomal dominant (ADFMF). ADFMF is characterized by periodic fever, serosal inflammation and pain in the abdomen, chest or joints as seen also in the autosomal recessive form of the disease. It is associated with renal amyloidosis and characterized by colchicine unresponsiveness. 2 isoforms of the human protein are produced by alternative splicing.
Cellular Component: microtubule; microtubule associated complex; cell projection; cytoskeleton; cytoplasm; intracellular; nucleus
Molecular Function: zinc ion binding; metal ion binding; actin binding
Biological Process: negative regulation of interleukin-12 production; immune system process; negative regulation of inflammatory response; innate immune response; negative regulation of interleukin-1 beta production; inflammatory response
Research Articles on MEFV
1. These results are consistent with a model in which pyrin acts to limit the release of IL-1beta generated by activation and assembly of inflammasomes in response to subclinical immune challenges.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
