Extracellular serine/threonine protein kinase FAM20C; Dentin matrix protein 4; DMP-4; Golgi-enriched fraction casein kinase; GEF-CK; Protein FAM20C; FAM20C; DMP4

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit Ig

Rabbit

This DMP4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 443-471 amino acids from the C-terminal region of human DMP4.

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Vial Concentration: 0.5 (lot specific)

Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Small volumes of anti-FAM20C antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Calcium-binding protein which may play a role in dentin mineralization (By similarity).

Developmental Biology; Signal Transduction

Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)

WB~~1:1000

DMP4 Antibody (C-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the DMP4 antibody detected the DMP4 protein (arrow).

DMP4 antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DMP4 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

66234

family with sequence similarity 20, member C

extracellular serine/threonine protein kinase FAM20C

Extracellular serine/threonine protein kinase FAM20C

DMP4; DMP-4; GEF-CK??[Similar Products]

DMP4_HUMAN

This gene encodes a member of the family of secreted protein kinases. The encoded protein binds calcium and phosphorylates proteins involved in bone mineralization. Mutations in this gene are associated with the autosomal recessive disorder Raine syndrome. [provided by RefSeq, Apr 2014]

FAM20C: an atypical serine protein kinase that localizes within the Golgi apparatus and is secreted. Distantly related to the Drosophila Golgi-localized kinase Four-jointed. Phosphorylates secretory proteins including the caseins and members of the small integrin-binding ligand, N-linked glycoprotein (SIBLING) protein family, which modulate biomineralization. Colocalizes with the Golgi resident protein GM130. Preferentially phosphorylates its targets within the S-x-E/pS motif. Requires Mn2+ as an alternative to Mg2+ as the activating cation. Defects in FAM20C are the cause of Raine syndrome (RNS). RNS is an autosomal recessive osteosclerotic bone dysplasia with neonatal lethal outcome. Clinical features include generalized osteosclerosis, craniofacial dysplasia and microcephaly. Two isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 7p22.3

Cellular Component: Golgi apparatus; extracellular space

Molecular Function: protein serine/threonine kinase activity; calcium ion binding

Biological Process: positive regulation of osteoblast differentiation; regulation of fibroblast growth factor receptor signaling pathway; positive regulation of bone mineralization; regulation of phosphorus metabolic process; protein amino acid phosphorylation

Disease: Raine Syndrome

Simpson, M.A., et al. Clin. Genet. 75(3):271-276(2009)
Simpson, M.A., et al. Am. J. Hum. Genet. 81(5):906-912(2007)
Hao, J., et al. J. Biol. Chem. 282(21):15357-15365(2007)
Nalbant, D., et al. BMC Genomics 6 (1), 11 (2005) :

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