AK 2; ATP-AMP transphosphorylase 2; ATP:AMP phosphotransferase; Adenylate monophosphate kinase; AK2; ADK2

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

IgG

Rabbit

Antigen Affinity Purified

Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Small volumes of anti-AK2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism. Adenylate kinase activity is critical for regulation of the phosphate utilization and the AMP de novo biosynthesis pathways. Plays a key role in hematopoiesis.

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

Recommended dilution: WB:1:500-2000
IHC:1:20-1:200

All lanes: Adenylate kinase 2, mitochondrial antibody at 5ug/ml
Lane 1:HeLa whole cell lysate
Lane 2:mouse kidney tissue
Lane 3:mouse small intestine tissue
Secondary
Goat polyclonal to rabbit at 1/10000 dilution
Predicted band size: 27,26,23,15,25,22 kDa
Observed band size: 26 kDa

Immunohistochemistry of paraffin-embedded human liver using MBS1496594 at dilution 1:100

Immunohistochemistry of paraffin-embedded human kidney using MBS1496594 at dilution 1:100

21,636 Da

adenylate kinase 2

adenylate kinase 2, mitochondrial

Adenylate kinase 2, mitochondrial

AK 2??[Similar Products]

KAD2_HUMAN

Adenylate kinases are involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate groups among adenine nucleotides. Three isozymes of adenylate kinase, namely 1, 2, and 3, have been identified in vertebrates; this gene encodes isozyme 2. Expression of these isozymes is tissue-specific and developmentally regulated. Isozyme 2 is localized in the mitochondrial intermembrane space and may play a role in apoptosis. Mutations in this gene are the cause of reticular dysgenesis. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 1 and 2.[provided by RefSeq, Nov 2010]

AK2: Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. This small ubiquitous enzyme involved in energy metabolism and nucleotide synthesis that is essential for maintenance and cell growth. Plays a key role in hematopoiesis. Defects in AK2 are the cause of reticular dysgenesis (RDYS); also known as aleukocytosis. RDYS is the most severe form of inborn severe combined immunodeficiencies (SCID) and is characterized by absence of granulocytes and almost complete deficiency of lymphocytes in peripheral blood, hypoplasia of the thymus and secondary lymphoid organs, and lack of innate and adaptive humoral and cellular immune functions, leading to fatal septicemia within days after birth. In bone marrow of individuals with reticular dysgenesis, myeloid differentiation is blocked at the promyelocytic stage, whereas erythro- and megakaryocytic maturation is generally normal.In addition, affected newborns have bilateral sensorineural deafness. Defects may be due to its absence in leukocytes and inner ear, in which its absence can not be compensated by AK1. Belongs to the adenylate kinase family. AK2 subfamily. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial; Nucleotide Metabolism - purine; EC 2.7.4.3; Kinase, other

Chromosomal Location of Human Ortholog: 1p34

Cellular Component: cytosol; mitochondrial inner membrane; mitochondrial intermembrane space

Molecular Function: adenylate kinase activity; ATP binding

Biological Process: ADP biosynthetic process; AMP metabolic process; brain development; dATP metabolic process; liver development; nucleobase, nucleoside and nucleotide interconversion; nucleobase, nucleoside and nucleotide metabolic process; nucleotide phosphorylation; oxidative phosphorylation

Disease: Reticular Dysgenesis

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