Anti -4E Binding Protein 1, non-phosphorylated (Thr46) (Protein Synthesis Initiation Factor 4E Binding Protein, eIF-4EBP1, PHAS-1, PHAS-I, Eukaryotic Translation Initiation Factor BP)

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 8; NC_000008.10 (37888020..37917883). Location: 8p12

Monoclonal

IgG

7H1

Rabbit

Detects endogenous levels of 4E-BP1 only when dephosphorylated at Thr46. Crossreacts with 4E-BP2 and 4E-BP3 dephosphorylated at equivalent sites.

Supernatant
Supernatant

Supplied as a liquid in 10mM sodium HEPES (pH 7.5), 150mM NaCl, 100ug/ml BSA, 50% glycerol and less than 0.02% sodium azide.

May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Small volumes of anti-EIF4EBP1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

4E-BP1 (also known as PHAS-1) when bound to eIF4E, inhibits cap-dependent translation. Upon hyperphosphorylation of 4E-BP1 this interaction is disrupted and cap-dependent translation is activated (1). Both the PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1 activity (2,3). Multiple 4E-BP1 residues are phosphorylated in vivo (4); while phosphorylation by FRAP/mTOR on Thr37 and Thr46 does not prevent the binding of 4E-BP-1 to eIF4E, it is thought to prime 4E-BP1 for subsequent phosphorylation at Ser65 and Thr70 (5).

Antibodies; Abs to Translation Initiation Factors

ELISA (EL/EIA), Western Blot (WB), Flow Cytometry (FC/FACS)

Suitable for use in Flow Cytometry, ELISA, Western Blot.
Dilution: Western blot 1:1000
Flow Cytometry 1:200

12,580 Da[Similar Products]

eukaryotic translation initiation factor 4E binding protein 1

eukaryotic translation initiation factor 4E-binding protein 1; OTTHUMP00000225572; eIF4E-binding protein 1; phosphorylated heat- and acid-stable protein regulated by insulin 1

Eukaryotic translation initiation factor 4E-binding protein 1

4EBP1_HUMAN

This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. [provided by RefSeq]

4E-BP1: binds to eIF4E, preventing its assembly into the EIF4F complex and inhibiting cap-dependent translation. Phosphorylation of 4E-BP1 disrupts this binding, activating cap-dependent translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the PI3 kinase pathway.

Protein type: Translation; Translation initiation

Chromosomal Location of Human Ortholog: 8p12

Cellular Component: nucleoplasm; protein complex; cytoplasm; cytosol

Molecular Function: protein binding; translation repressor activity; eukaryotic initiation factor 4E binding

Biological Process: negative regulation of translational initiation; response to ethanol; TOR signaling pathway; cellular protein metabolic process; translation; translational initiation; insulin receptor signaling pathway; gene expression; positive regulation of mitotic cell cycle; negative regulation of protein complex assembly; lung development; G1/S transition of mitotic cell cycle

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