Product Name
CANT1, Recombinant Protein
Full Product Name
CANT1, 63-401aa, Human, His tag, E Coli
Product Synonym Names
Soluble calcium-activated nucleotidase 1; DBQD; SCAN-1; SCAN1; SHAPY
Product Gene Name
CANT1 recombinant protein
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMRPAPG RPPTHNAHNW RLGQAPANWY NDTYPLSPPQ RTPAGIRYRI AVIADLDTES RAQEENTWFS YLKKGYLTLS DSGDKVAVEW DKDHGVLESH LAEKGRGMEL SDLIVFNGKL YSVDDRTGVV YQIEGSKAVP WVILSDGDGT VEKGFKAEWL AVKDERLYVG GLGKEWTTTT GDVVNENPEW VKVVGYKGSV DHENWVSNYN ALRAAAGIQP PGYLIHESAC WSDTLQRWFF LPRRASQERY SEKDDERKGA NLLLSASPDF GDIAVSHVGA VVPTHGFSSF KFIPNTDDQI IVALKSEEDS GRVASYIMAF TLDGRFLLPE TKIGSVKYEG IEFI
3D Structure
ModBase 3D Structure for Q8WVQ1
Purity/Purification
> 90% by SDS-PAGE
Form/Format
Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 50mM NaCl
Concentration
1mg/ml (determined by Bradford assay) (lot specific)
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
Other Notes
Small volumes of CANT1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
CANT1 recombinant protein
CANT1 (calcium-activated nucleotidase 1) belongs to the apyrase family. This protein is calcium-dependent nucleotidase with a preference for uDP. The order of activity with different substrates is uDP > GDP > uTP > GTP. Also, it has very low activity towards ADP and even lower activity towards ATP. And it does not hydrolyze AMP and GMP. The specific function of CANT1 is as yet unknown, but its substrates are involved in several major signaling functions, including Ca2+ release, through activation of pyrimidinergic signaling. Recombinant human CANT1 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.
Product Categories/Family for CANT1 recombinant protein
Signal Transduction
Applications Tested/Suitable for CANT1 recombinant protein
SDS-PAGE
SDS-Page of CANT1 recombinant protein
NCBI/Uniprot data below describe general gene information for CANT1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_620148
[Other Products]
NCBI GenBank Nucleotide #
NM_138793.3
[Other Products]
UniProt Primary Accession #
Q8WVQ1
[Other Products]
UniProt Secondary Accession #
Q7Z2J7; Q8NG05; Q8NHP0; Q9BSD5; B4DJ54[Other Products]
UniProt Related Accession #
Q8WVQ1[Other Products]
Molecular Weight
40.5kDa (364aa), confirmed by MALDI-TOF
NCBI Official Full Name
soluble calcium-activated nucleotidase 1
NCBI Official Synonym Full Names
calcium activated nucleotidase 1
NCBI Official Symbol
CANT1??[Similar Products]
NCBI Official Synonym Symbols
DBQD; EDM7; DBQD1; SCAN1; SHAPY; SCAN-1
??[Similar Products]
NCBI Protein Information
soluble calcium-activated nucleotidase 1
UniProt Protein Name
Soluble calcium-activated nucleotidase 1
UniProt Synonym Protein Names
Apyrase homolog; Putative MAPK-activating protein PM09; Putative NF-kappa-B-activating protein 107
Protein Family
Soluble calcium-activated nucleotidase
UniProt Gene Name
CANT1??[Similar Products]
UniProt Synonym Gene Names
SHAPY; SCAN-1??[Similar Products]
NCBI Summary for CANT1
This protein encoded by this gene belongs to the apyrase family. It functions as a calcium-dependent nucleotidase with a preference for UDP. Mutations in this gene are associated with Desbuquois dysplasia with hand anomalies. Alternatively spliced transcript variants have been noted for this gene.[provided by RefSeq, Mar 2010]
UniProt Comments for CANT1
Calcium-dependent nucleotidase with a preference for UDP. The order of activity with different substrates is UDP > GDP > UTP > GTP. Has very low activity towards ADP and even lower activity towards ATP. Does not hydrolyze AMP and GMP (PubMed:12234496, PubMed:15248776, PubMed:15006348, PubMed:16835225). Involved in proteoglycan synthesis (PubMed:22539336).
Product References and Citations for CANT1 recombinant protein
Yang M., et al. (2004) Biochemistry 43:9185-9194; Huber C, et al. (2009) Am J Hum Genet. 85 (5):706-10.
Research Articles on CANT1
1. The Multiple Epiphyseal Dysplasia (MED)phenotype is thus allelic to the more severe Desbuquois dysplasia phenotype and the results identify CANT1 as a second locus for recessively inherited MED
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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