Product Name
Collagen Type VI (COL6A1), Antibody
Full Product Name
Collagen Type VI, human
Product Gene Name
anti-COL6A1 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P12109
Species Reactivity
Human, Bovine, Porcine, Chicken, Murine, Rat tissue
Purity/Purification
Affinity Purified
Form/Format
Lyophilized from phosphate buffered solution; no BSA and preservative added.
Concentration
1 mg/ml (lot specific)
Immunogen
Purified collagen type VI from human placenta
Positive Control
Human placenta
Incubation Time
IHC(P) 60 min at RT or 2-8 degree C over night
Pre-Treatment
After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 min at 37 degree C. Thereafter non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.
Preparation and Storage
Store at -20 degree C.
Other Notes
Small volumes of anti-COL6A1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-COL6A1 antibody
Collagen type VI is found as 5-10 nm diameter microfibrils (periodicity 100 nm) e.g. in placenta, skin, cartilage and cornea. Collagens consist of a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule consists of a triple-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Human collagen type VI 100%, human collagen type I, II, III, IV and V <0.1%; human elastin and human laminin <0.1%; human fibronectin < 1% (RIA at 1:10.000 dilution).
Applications Tested/Suitable for anti-COL6A1 antibody
Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF), ELISA (EIA), Radioimmunoassay (RIA), Immunoblotting (IB), Western Blot (WB)
Application Notes for anti-COL6A1 antibody
IFA: 1:80
IHC-P: 1:1000
ELISA: 1:200
NCBI/Uniprot data below describe general gene information for COL6A1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001839.2
[Other Products]
NCBI GenBank Nucleotide #
NM_001848.2
[Other Products]
UniProt Primary Accession #
P12109
[Other Products]
UniProt Secondary Accession #
O00117; O00118; Q14040; Q14041; Q16258; Q7Z645; Q9BSA8[Other Products]
UniProt Related Accession #
P12109[Other Products]
Molecular Weight
108,529 Da[Similar Products]
NCBI Official Full Name
collagen alpha-1(VI) chain
NCBI Official Synonym Full Names
collagen type VI alpha 1 chain
NCBI Official Symbol
COL6A1??[Similar Products]
NCBI Official Synonym Symbols
OPLL; BTHLM1; UCHMD1
??[Similar Products]
NCBI Protein Information
collagen alpha-1(VI) chain
UniProt Protein Name
Collagen alpha-1(VI) chain
UniProt Gene Name
COL6A1??[Similar Products]
NCBI Summary for COL6A1
The collagens are a superfamily of proteins that play a role in maintaining the integrity of various tissues. Collagens are extracellular matrix proteins and have a triple-helical domain as their common structural element. Collagen VI is a major structural component of microfibrils. The basic structural unit of collagen VI is a heterotrimer of the alpha1(VI), alpha2(VI), and alpha3(VI) chains. The alpha2(VI) and alpha3(VI) chains are encoded by the COL6A2 and COL6A3 genes, respectively. The protein encoded by this gene is the alpha 1 subunit of type VI collagen (alpha1(VI) chain). Mutations in the genes that code for the collagen VI subunits result in the autosomal dominant disorder, Bethlem myopathy. [provided by RefSeq, Jul 2008]
UniProt Comments for COL6A1
Collagen VI acts as a cell-binding protein.
Product References and Citations for anti-COL6A1 antibody
1. Ronzière M.C., Ricard-Blum S., Tiollier J., Hartmann D.J., Garrone R., Herbage D. (1990) Comparative analysis of collagens solubilized from human foetal, and normal and osteoarthritic ***** articular cartilage, with emphasis on type VI collagen. Biochim Biophys Acta. Apr 19;1038(2):222-30. 2. Silvester LM, Luck MR (1999) Distribution of extracellular matrix components in the developing ruminant corpus luteum: a wound repair hypothesis for luteinization. J Reprod Fertil. May;116(1):187-98.
Research Articles on COL6A1
1. Missense mutations in COL6A1, COL11A2, FGFR1, and BMP2 genetically predispose patients to ossification of posterior longitudinal ligaments.
Precautions
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Disclaimer
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