For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of Rat ABRA. No significant cross-reactivity or interference between Rat ABRA and analogues was observed.

Store at 2-8 degree C.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of ABRA elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS280982 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Actin-binding Rho-activating protein (ABRA) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ABRA. The ELISA analytical biochemical technique of the MBS280982 kit is based on ABRA antibody-ABRA antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ABRA antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ABRA. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate ABRA in samples. An antibody specific for ABRA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyABRA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ABRA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ABRA bound in the initial step. The color development is stopped and the intensity of the color is measured.

42,901 Da

actin-binding Rho activating protein

actin-binding Rho-activating protein

Actin-binding Rho-activating protein

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ABRA: Acts as an activator of serum response factor (SRF)- dependent transcription possibly by inducing nuclear translocation of MKL1 or MKL2 and through a mechanism requiring Rho-actin signaling.

Protein type: Actin-binding; Contractile; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 7q31

Cellular Component: actin cytoskeleton; myofibril; plasma membrane; sarcomere

Molecular Function: actin binding

Biological Process: positive regulation of Rho protein signal transduction; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-templated; protein import into nucleus, translocation; transcription, DNA-dependent

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