Product Name
ZFYVE26, siRNA
Full Product Name
ZFYVE26 siRNA (Human)
Product Synonym Names
KIAA0321; Zinc finger FYVE domain-containing protein 26; FYVE domain-containing centrosomal protein; FYVE-CENT; Spastizin
Product Gene Name
ZFYVE26 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q68DK2
Specificity
ZFYVE26 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ZFYVE26 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of ZFYVE26 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
ZFYVE26 sirna
siRNA to inhibit ZFYVE26 expression using RNA interference
Applications Tested/Suitable for ZFYVE26 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for ZFYVE26. It may not necessarily be applicable to this product.
NCBI Accession #
NP_056161.2
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NCBI GenBank Nucleotide #
NM_015346.3
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UniProt Primary Accession #
Q68DK2
[Other Products]
UniProt Secondary Accession #
O15035; Q68DT9; Q6AW90; Q6ZR50; Q7Z3A4; Q7Z3I1; Q8N4W7; B1B5Y3; B4E2U3[Other Products]
UniProt Related Accession #
Q68DK2[Other Products]
Molecular Weight
77,970 Da
NCBI Official Full Name
zinc finger FYVE domain-containing protein 26
NCBI Official Synonym Full Names
zinc finger, FYVE domain containing 26
NCBI Official Symbol
ZFYVE26??[Similar Products]
NCBI Official Synonym Symbols
SPG15; FYVE-CENT
??[Similar Products]
NCBI Protein Information
zinc finger FYVE domain-containing protein 26
UniProt Protein Name
Zinc finger FYVE domain-containing protein 26
UniProt Synonym Protein Names
FYVE domain-containing centrosomal protein; FYVE-CENT; Spastizin
Protein Family
Zinc finger FYVE domain-containing protein
UniProt Gene Name
ZFYVE26??[Similar Products]
UniProt Synonym Gene Names
KIAA0321; FYVE-CENT??[Similar Products]
UniProt Entry Name
ZFY26_HUMAN
NCBI Summary for ZFYVE26
This gene encodes a protein which contains a FYVE zinc finger binding domain. The presence of this domain is thought to target these proteins to membrane lipids through interaction with phospholipids in the membrane. Mutations in this gene are associated with autosomal recessive spastic paraplegia-15. [provided by RefSeq, Oct 2008]
UniProt Comments for ZFYVE26
ZFYVE26: Phosphatidylinositol 3-phosphate-binding protein required for the abcission step in cytokinesis: recruited to the midbody during cytokinesis and acts as a regulator of abcission. May also be required for efficient homologous recombination DNA double-strand break repair. Interacts with AP5Z1, AP5B1, AP5S1 and SPG11. Interacts with TTC19 and KIF13A. Strongest expression in the adrenal gland, bone marrow, ***** brain, fetal brain, lung, placenta, prostate, skeletal muscle, testis, thymus, and retina. Intermediate levels are detected in other structures, including the spinal cord. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Unknown function
Chromosomal Location of Human Ortholog: 14q24.1
Cellular Component: centrosome; lysosomal membrane; midbody
Molecular Function: protein binding; metal ion binding; phosphatidylinositol 3-phosphate binding
Biological Process: cytokinesis; double-strand break repair via homologous recombination
Disease: Spastic Paraplegia 15, Autosomal Recessive
Research Articles on ZFYVE26
1. spastizin and spatacsin were essential components for the initiation of lysosomal tubulation. Together, these results link dysfunction of the autophagy/lysosomal biogenesis machinery to neurodegeneration.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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