Human Melanotransferrin (MFI2) ELISA kit; CD228; FLJ38863; MAP97; MGC4856; MTF1; melanoma-associated antigen p97; melanoma-associated antigen p97; isoform 2; melanotransferrin; membrane-bound transferrin-like protein; antigen p97 (melanoma associated) identified by monoclonal antibodies 133.2 and 96.5

For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of Human MFI2. No significant cross-reactivity or interference between Human MFI2 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of MFI2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS946083 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the antigen p97 (melanoma associated) identified by monoclonal antibodies 133.2 and 96.5 (MFI2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MFI2. The ELISA analytical biochemical technique of the MBS946083 kit is based on MFI2 antibody-MFI2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MFI2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MFI2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for melanotransferrin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any melanotransferrin present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for melanotransferrin is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of melanotransferrin bound in the initial step. The color development is stopped and the intensity of the color is measured.

80,215 Da

antigen p97 (melanoma associated) identified by monoclonal antibodies 133.2 and 96.5

melanotransferrin; membrane-bound transferrin-like protein; melanoma-associated antigen p97, isoform 2

Melanotransferrin

MAP97??[Similar Products]

TRFM_HUMAN

The protein encoded by this gene is a cell-surface glycoprotein found on melanoma cells. The protein shares sequence similarity and iron-binding properties with members of the transferrin superfamily. The importance of the iron binding function has not yet been identified. This gene resides in the same region of chromosome 3 as members of the transferrin superfamily. Alternative splicing results in two transcript variants. [provided by RefSeq, Jul 2008]

MFI2: Involved in iron cellular uptake. Seems to be internalized and then recycled back to the cell membrane. Binds a single atom of iron per subunit. Could also bind zinc. Belongs to the transferrin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 3q28-q29

Cellular Component: anchored to plasma membrane; cell surface; integral to plasma membrane

Molecular Function: protein binding; ferric iron binding; iron ion binding

Biological Process: cellular iron ion homeostasis; regulation of cell growth; regulation of cell proliferation

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