For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

IgG

Rabbit

The antibody is a rabbit polyclonal antibody raised against ENPP1. It has been selected for its ability to recognize ENPP1 in immunohistochemical staining andwestern blotting.

Affinity Chromatography

Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.

200ug/ml (lot specific)

Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.

Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customera??s specifications, please inquire.

Small volumes of anti-ENPP1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)

Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200

Western Blot: Sample: Rat Kidney lysate; Primary Ab: 1ug/ml Rabbit Anti-Rat ENPP1 Antibody Second Ab: 0.2ug/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody

Western Blot: Sample: Recombinant protein.

DAB staining on fromalin fixed paraffin- embedded stomach tissue)

DAB staining on IHC-P; Samples: Rat Ovary Tissue)

102,843 Da

ectonucleotide pyrophosphatase/phosphodiesterase 1

ectonucleotide pyrophosphatase/phosphodiesterase family member 1

Ectonucleotide pyrophosphatase/phosphodiesterase family member 1

Npps; Pc1; Pdnp1; NPPase??[Similar Products]

mutation of the human homolog is detected in patients with idiopathic infantile arterial calcification [RGD, Feb 2006]

ENPP1: Involved primarily in ATP hydrolysis at the plasma membrane. Plays a role in regulating pyrophosphate levels, and functions in bone mineralization and soft tissue calcification. In vitro, has a broad specificity, hydrolyzing other nucleoside 5' triphosphates such as GTP, CTP, TTP and UTP to their corresponding monophosphates with release of pyrophosphate and diadenosine polyphosphates, and also 3',5'-cAMP to AMP. May also be involved in the regulation of the availability of nucleotide sugars in the endoplasmic reticulum and Golgi, and the regulation of purinergic signaling. Appears to modulate insulin sensitivity. Homodimer; disulfide-linked. Interacts with INSR. Expressed in plasma cells and also in a number of non-lymphoid tissues, including the distal convoluted tubule of the kidney, chondrocytes and epididymis. At low concentrations of ATP, a phosphorylated intermediate is formed which inhibits further hydrolysis. Belongs to the nucleotide pyrophosphatase/phosphodiesterase family.

Protein type: Carbohydrate Metabolism - starch and sucrose; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; Cofactor and Vitamin Metabolism - pantothenate and CoA biosynthesis; Cofactor and Vitamin Metabolism - riboflavin; EC 3.1.4.1; EC 3.6.1.9; Membrane protein, integral; Motility/polarity/chemotaxis; Nucleotide Metabolism - purine; Phosphatase (non-protein); Phosphodiesterase

Chromosomal Location of Human Ortholog: 1p12

Cellular Component: basolateral plasma membrane; cell body; cell projection; cell soma; cell surface; extracellular region; extracellular space; integral component of membrane; integral component of plasma membrane; lysosomal membrane; neuron projection; plasma membrane; synapse

Molecular Function: ATP binding; calcium ion binding; exonuclease activity; insulin receptor binding; metal ion binding; nucleic acid binding; nucleoside-triphosphate diphosphatase activity; nucleotide diphosphatase activity; phosphodiesterase I activity; polysaccharide binding; protein homodimerization activity; scavenger receptor activity; zinc ion binding

Biological Process: 3'-phosphoadenosine 5'-phosphosulfate metabolic process; ATP metabolic process; biomineral formation; bone remodeling; cellular phosphate ion homeostasis; cellular response to acidic pH; cellular response to cAMP; cellular response to insulin stimulus; cellular response to transforming growth factor beta stimulus; cerebellum development; cerebral cortex development; generation of precursor metabolites and energy; hippocampus development; immune response; inorganic diphosphate transport; liver development; negative regulation of cell growth; negative regulation of fat cell differentiation; negative regulation of glucose import; negative regulation of glycogen biosynthetic process; negative regulation of insulin receptor signaling pathway; negative regulation of ossification; negative regulation of protein autophosphorylation; nucleic acid phosphodiester bond hydrolysis; nucleoside triphosphate catabolic process; olfactory bulb development; phosphate-containing compound metabolic process; receptor-mediated endocytosis; response to mechanical stimulus; sequestering of triacylglycerol; striatum development

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