Product Name
PLEKHM1, siRNA
Full Product Name
PLEKHM1 siRNA (Mouse)
Product Synonym Names
Pleckstrin homology domain-containing family M member 1; PH domain-containing family M member 1
Product Gene Name
PLEKHM1 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q7TSI1
Specificity
PLEKHM1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse PLEKHM1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of PLEKHM1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
PLEKHM1 sirna
siRNA to inhibit PLEKHM1 expression using RNA interference
Applications Tested/Suitable for PLEKHM1 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for PLEKHM1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_898855.1
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NCBI GenBank Nucleotide #
NM_183034.1
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UniProt Primary Accession #
Q7TSI1
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UniProt Secondary Accession #
Q3U3L2; Q8CHU5[Other Products]
UniProt Related Accession #
Q7TSI1[Other Products]
Molecular Weight
118,535 Da
NCBI Official Full Name
pleckstrin homology domain-containing family M member 1
NCBI Official Synonym Full Names
pleckstrin homology domain containing, family M (with RUN domain) member 1
NCBI Official Symbol
Plekhm1??[Similar Products]
NCBI Official Synonym Symbols
B2; AP162; BC038943; D330036J23Rik
??[Similar Products]
NCBI Protein Information
pleckstrin homology domain-containing family M member 1
UniProt Protein Name
Pleckstrin homology domain-containing family M member 1
Protein Family
Pleckstrin homology domain-containing family
UniProt Gene Name
Plekhm1??[Similar Products]
UniProt Synonym Gene Names
PH domain-containing family M member 1??[Similar Products]
UniProt Entry Name
PKHM1_MOUSE
UniProt Comments for PLEKHM1
PLEKHM1: Involved in vesicular transport in the osteoclast. May have a role in sialyl-lex-mediated transduction of apoptotic signals. Defects in PLEKHM1 are the cause of osteopetrosis autosomal recessive type 6 (OPTB6); also known as autosomal recessive osteopetrosis intermediate form. Osteopetrosis is a rare genetic disease characterized by abnormally dense bone, due to defective resorption of immature bone. The disorder occurs in two forms: a severe autosomal recessive form occurring in utero, infancy, or childhood, and a benign autosomal dominant form occurring in adolescence or *****hood. Autosomal recessive osteopetrosis is usually associated with normal or elevated amount of non-functional osteoclasts.
Protein type: Unknown function
Cellular Component: cytoplasm
Molecular Function: protein binding; metal ion binding
Research Articles on PLEKHM1
1. results indicate that LIS1 is a previously unrecognized regulator of osteoclast formation, microtubule organization, and lysosomal secretion by virtue of its ability to modulate dynein function and Plekhm1
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
