RP11-459F1.1; MGC104817; TFT; OTTHUMP00000017588|T brachyury homolog|T brachyury-like|transcription factor T

For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of Mouse T. No significant cross-reactivity or interference between Mouse T and analogues was observed.

Store at 2-8 degree C.

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Small volumes of T elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS284314 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Brachyury (T) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing T. The ELISA analytical biochemical technique of the MBS284314 kit is based on T antibody-T antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect T antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, T. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate T in samples. An antibody specific for T has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for T is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of T bound in the initial step. The color development is stopped and the intensity of the color is measured.

47,440 Da

brachyury, T-box transcription factor T

brachyury protein

Brachyury protein

Brachyury: Involved in the transcriptional regulation of genes required for mesoderm formation and differentiation. Binds to a palindromic site (called T site) and activates gene transcription when bound to such a site. Genetic variations in T are associated with susceptibility to neural tube defects (NTD). NTD are common congenital malformations. Spina bifida, which results from malformations in the caudal region of the neural tube, is compatible with life but associated with significant morbidity, including lower limb paralysis. T is involved in susceptibility to the development of chordoma (CHDM). Chordomas are rare, clinically malignant tumors derived from notochordal remnants. They occur along the length of the spinal axis, predominantly in the sphenooccipital, vertebral and sacrococcygeal regions. They are characterized by slow growth, local destruction of bone, extension into adjacent soft tissues and rarely, distant metastatic spread. Susceptibility to development of chordomas is due to a T gene duplication.

Protein type: Cell cycle regulation; DNA-binding; Motility/polarity/chemotaxis; Transcription factor

Chromosomal Location of Human Ortholog: 17 A1|17 4.92 cM

Cellular Component: chromatin; cytoplasm; nuclear chromatin; nucleus

Molecular Function: DNA binding; DNA binding transcription factor activity; protein binding; sequence-specific DNA binding; transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding

Biological Process: anatomical structure morphogenesis; anterior/posterior pattern specification; BMP signaling pathway; cardiac muscle cell differentiation; embryonic skeletal system development; heart morphogenesis; mesoderm development; mesoderm migration involved in gastrulation; multicellular organism development; negative regulation of transcription from RNA polymerase II promoter; neural plate morphogenesis; neural tube closure; notochord development; notochord formation; penetration of zona pellucida; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-templated; reproductive process; somitogenesis; transcription, DNA-dependent; vasculogenesis; Wnt receptor signaling pathway through beta-catenin

Disease: Neural Tube Defects

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