Anti -PTF1A (Pancreas Transcription Factor 1 Subunit alpha, Class A Basic Helix-loop-helix protein 29, bHLHa29, Pancreas-specific Transcription Factor 1a, bHLH Transcription Factor p48, p48 DNA-binding Subunit of Transcription Factor PTF1, PTF1-p48, BHLHA29, PT

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 10; NC_000010.10 (23481460..23483181). Location: 10p12.2

Polyclonal

IgG

Goat

Recognizes human PTF1A. Species sequence homology: mouse (88%).

Affinity Purified
Purified by immunoaffinity chromatography.

Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with sterile PBS.

Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Small volumes of anti-PTF1A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

PTF1A (Pancreas transcription factor 1a; also p48 and bHLHA29) is a cell specific member of the bHLH family of proteins. Although its predicted MW is 41kD, a high ratio of Pro, Leu and Ala increases its MW to 48kD in SDS-PAGE. PTF1A forms a trimeric transcription factor complex with a p75 subunit (HEB, E2 or E47) and a p64 subunit (usually RBP-L). Within this complex, unfortunately termed PTF1, p48/PTF1A binds to a DNA E-box while p64 binds to a TC-box. This regulates the gene transcription of about 20 enzymes synthesized by pancreatic acinar cells. Human PTF1A is 328aa in length. It contains one basic DNA binding HLH motif (aa150-175) and a dimerization interface (aa178-219). Overaa1-92, human PTF1A shares 88% aa identity with mouse PTF1A.

Antibodies; Abs to Transcription Factors

ELISA (EL/EIA), Western Blot (WB)

Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 0.5-1ug/ml
Western Blot: 1ug/ml

34,970 Da[Similar Products]

pancreas specific transcription factor, 1a

pancreas transcription factor 1 subunit alpha; OTTHUMP00000019309; class II bHLH protein PTF1A; bHLH transcription factor p48; class A basic helix-loop-helix protein 29; pancreas-specific transcription factor 1a; exocrine pancreas-specific transcription factor p48; p48 DNA-binding subunit of transcription factor PTF1

Pancreas transcription factor 1 subunit alpha

BHLHA29; PTF1P48??[Similar Products]

PTF1A_HUMAN

This gene encodes a protein that is a component of the pancreas transcription factor 1 complex (PTF1) and is known to have a role in mammalian pancreatic development. The protein plays a role in determining whether cells allocated to the pancreatic buds continue towards pancreatic organogenesis or revert back to duodenal fates. The protein is thought to be involved in the maintenance of exocrine pancreas-specific gene expression including elastase 1 and amylase. Mutations in this gene cause cerebellar agenesis and loss of expression is seen in ductal type pancreas cancers. [provided by RefSeq]

PTF1A: Transcriptional activator. Binds to the E-box consensus sequence 5'-CANNTG-3'. Plays an important role in determining whether cells allocated to the pancreatic buds continue towards pancreatic organogenesis or revert back to duodenal fates. May be involved in the maintenance of exocrine pancreas-specific gene expression including ELA1 and amylase. Required for the formation of pancreatic acinar and ductal cells. Plays an important role in cerebellar development. Defects in PTF1A are the cause of diabetes mellitus and cerebellar hypoplasia/agenesis (DMCH).

Chromosomal Location of Human Ortholog: 10p12.2

Cellular Component: transcription factor complex; cytoplasm; nucleus

Molecular Function: protein dimerization activity; DNA binding; sequence-specific DNA binding; chromatin binding

Biological Process: retinoic acid receptor signaling pathway; tissue development; embryonic development; transcription, DNA-dependent; regulation of transcription, DNA-dependent; pancreas development; neuron fate commitment; exocrine pancreas development; cerebellum development; positive regulation of transcription from RNA polymerase II promoter; endocrine pancreas development

Disease: Pancreatic And Cerebellar Agenesis; Pancreatic Agenesis 2

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