Transcription factor MafA; Pancreatic beta-cell-specific transcriptional activator; Transcription factor RIPE3b1; V-maf musculoaponeurotic fibrosarcoma oncogene homolog A; MAFA

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit Ig

Rabbit

This MAFA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 282-311 amino acids from the C-terminal region of human MAFA.

Purified Rabbit Polyclonal Antibody (Pab)

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Vial Concentration: 2 (lot specific)

Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Small volumes of anti-MAFA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MAFA is a transcription factor that binds RIPE3b, a conserved enhancer element that regulates pancreatic beta cell-specific expression of the insulin gene.

Cancer; Signal Transduction

Western Blot (WB), ELISA (EIA)

WB~~1:1000

Western blot analysis of MAFA Antibody (C-term) in WiDr cell line lysates (35ug/lane). MAFA (arrow) was detected using the purified Pab.

36982

v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog A

transcription factor MafA

Transcription factor MafA

MAFA_HUMAN

MAFA is a transcription factor that binds RIPE3b, a conserved enhancer element that regulates pancreatic beta cell-specific expression of the insulin gene (INS; MIM 176730) (Olbrot et al., 2002 [PubMed 12011435]).[supplied by OMIM, Mar 2008]

MAFA: Acts as a transcriptional factor. Specifically binds the insulin enhancer element RIPE3b and activates insulin gene expression. Cooperates synergistically with NEUROD1 and PDX1. Phosphorylation by GSK3 increases its transcriptional activity and is required for its oncogenic activity. Involved either as an oncogene or as a tumor suppressor, depending on the cell context. Binds DNA as a homodimer (Probable). Interacts with NEUROD1, PCAF and PDX1. Up-regulated by glucose. Belongs to the bZIP family. Maf subfamily.

Protein type: DNA-binding; Tumor suppressor; Transcription factor; Oncoprotein

Chromosomal Location of Human Ortholog: 8q24.3

Cellular Component: nucleus

Molecular Function: protein homodimerization activity; DNA binding; protein heterodimerization activity; sequence-specific DNA binding; transcription factor activity

Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent; insulin secretion; response to glucose stimulus; positive regulation of transcription from RNA polymerase II promoter; nitric oxide mediated signal transduction

Kondo, T., et al. Mol. Endocrinol. 23(8):1281-1290(2009)
Kaneto, H., et al. Adv. Drug Deliv. Rev. 61 (7-8), 489-496 (2009)
Garin, I., et al. Diabet. Med. 26(7):746-748(2009)
Vanderford, N.L., et al. Arch. Biochem. Biophys. 480(2):138-142(2008)
Martin, C.C., et al. J. Mol. Endocrinol. 41(5):315-328(2008)

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