For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

IgG

Rabbit

Affinity Purification

Store at -20 degree C. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Small volumes of anti-FTSJ1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

This gene encodes a member of the methyltransferase superfamily. The encoded protein localizes to the nucleolus, binds to S-adenosylmethionine, and may be involved in the processing and modification of ribosomal RNA. Mutations in this gene are associated with mental retardation. Alternative splicing results in multiple transcript variants.

Western Blot (WB), Immunohistochemistry (IHC)

WB 1:500 - 1:2000
IHC 1:50 - 1:200

Western blot analysis of extracts of HT-1080 cells, using FTSJ1 Antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.

36kDa

FtsJ RNA methyltransferase homolog 1 (E. coli)

putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase

Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase

TRM7_HUMAN

This gene encodes a member of the methyltransferase superfamily. The encoded protein localizes to the nucleolus, binds to S-adenosylmethionine, and may be involved in the processing and modification of ribosomal RNA. Mutations in this gene are associated with mental retardation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]

FTSJ1: Defects in FTSJ1 are the cause of mental retardation X- linked type 44 (MRX44). Mental retardation is characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. Non-syndromic mental retardation patients do not manifest other clinical signs. Belongs to the methyltransferase superfamily. RlmE family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Methyltransferase; EC 2.1.1.205

Chromosomal Location of Human Ortholog: Xp11.23

Cellular Component: cytoplasm

Molecular Function: tRNA methyltransferase activity

Disease: Mental Retardation, X-linked 9

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