Full Product Name
PRLH siRNA (Human)
Product Synonym Names
PRH; Prolactin-releasing peptide; PrRP; Prolactin-releasing hormone
Product Gene Name
PRLH sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P81277
Specificity
PRLH siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human PRLH gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of PRLH sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
PRLH sirna
siRNA to inhibit PRLH expression using RNA interference
Applications Tested/Suitable for PRLH sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for PRLH. It may not necessarily be applicable to this product.
NCBI Accession #
NP_056977.1
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NCBI GenBank Nucleotide #
NM_015893.1
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UniProt Primary Accession #
P81277
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UniProt Related Accession #
P81277[Other Products]
Molecular Weight
9,639 Da
NCBI Official Full Name
prolactin-releasing peptide
NCBI Official Synonym Full Names
prolactin releasing hormone
NCBI Official Symbol
PRLH??[Similar Products]
NCBI Official Synonym Symbols
PRH; PRRP
??[Similar Products]
NCBI Protein Information
prolactin-releasing peptide
UniProt Protein Name
Prolactin-releasing peptide
UniProt Synonym Protein Names
Prolactin-releasing hormone
Protein Family
Prolactin-releasing peptide
UniProt Gene Name
PRLH??[Similar Products]
UniProt Synonym Gene Names
PRH; PrRP??[Similar Products]
UniProt Entry Name
PRRP_HUMAN
UniProt Comments for PRLH
PRLH: Stimulates prolactin (PRL) release and regulates the expression of prolactin through its receptor GPR10. May stimulate lactotrophs directly to secrete PRL.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 2q37.3
Cellular Component: cytoplasm; extracellular region
Molecular Function: neuropeptide hormone activity; hormone activity; prolactin-releasing peptide receptor binding
Biological Process: fat cell differentiation; G-protein coupled receptor protein signaling pathway; autonomic nervous system development; energy reserve metabolic process; tissue homeostasis; response to glucose stimulus; regulation of multicellular organism growth; lipid metabolic process; reduction of food intake in response to dietary excess; response to insulin stimulus
Research Articles on PRLH
1. Review discusses the ancestral relationship between prolactin-releasing hormone and the C-terminal RF-motif amide precursor peptide (C-RF).
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
