Human CILP; Cartilage intermediate layer protein 1; CILP-1; Cartilage intermediate-layer protein; CILP; UNQ602/PRO1188; Cartilage intermediate layer protein

For Research Use Only. Not for use in diagnostic procedures.

Natural and recombinant human CILP

Store at 4 degree C for 6 months. Store at -20 degree C for 12 months.
Avoid multiple freeze-thaw cycles.

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

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Small volumes of CILP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS1751727 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the CILP, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CILP. The ELISA analytical biochemical technique of the MBS1751727 kit is based on CILP antibody-CILP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CILP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CILP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Description: For quantitative detection of human CILP in cell culture supernates, serum and plasma(heparin, EDTA, citrate).
Principle: The human CILP ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from human specific for CILP has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: M1-R720) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CILP is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substmousee TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Fibronectin amount of sample captured in plate.
Background: Cartilage intermediate layer protein 1 is a protein that in humans is encoded by the CILP gene. It is mapped to 15q22.31. Major alterations in the composition of the cartilage extracellular matrix occur in joint disease, such as osteoarthrosis. This gene encodes the cartilage intermediate layer protein (CILP), which increases in early osteoarthrosis cartilage. The encoded protein was thought to encode a protein precursor for two different proteins; an N-terminal CILP and a C-terminal homolog of NTPPHase, however, later studies identified no nucleotide pyrophosphatase phosphodiesterase (NPP) activity. The full-length and the N-terminal domain of this protein was shown to function as an IGF-1 antagonist. An allelic variant of this gene has been associated with lumbar disc disease.

Human CILP PicoKine ELISA Kit Standard Curve

132,565 Da

cartilage intermediate layer protein

cartilage intermediate layer protein 1

Cartilage intermediate layer protein 1

CILP-1??[Similar Products]

Major alterations in the composition of the cartilage extracellular matrix occur in joint disease, such as osteoarthrosis. This gene encodes the cartilage intermediate layer protein (CILP), which increases in early osteoarthrosis cartilage. The encoded protein was thought to encode a protein precursor for two different proteins; an N-terminal CILP and a C-terminal homolog of NTPPHase, however, later studies identified no nucleotide pyrophosphatase phosphodiesterase (NPP) activity. The full-length and the N-terminal domain of this protein was shown to function as an IGF-1 antagonist. An allelic variant of this gene has been associated with lumbar disc disease. [provided by RefSeq, Sep 2010]

Probably plays a role in cartilage scaffolding. May act by antagonizing TGF-beta1 (TGFB1) and IGF1 functions. Has the ability to suppress IGF1-induced proliferation and sulfated proteoglycan synthesis, and inhibits ligand-induced IGF1R autophosphorylation. May inhibit TGFB1-mediated induction of cartilage matrix genes via its interaction with TGFB1. Overexpression may lead to impair chondrocyte growth and matrix repair and indirectly promote inorganic pyrophosphate (PPi) supersaturation in aging and osteoarthritis cartilage.

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