Product Name
Poliovirus Receptor Related Protein 1 (PVRL1), ELISA Kit
Full Product Name
Human Poliovirus Receptor Related Protein 1 (PVRL1) ELISA Kit
Product Synonym Names
CD111; HVEC; PRR1; CLPED1; ED4; HIgR; OFC7; PRR; PVRR1; SK12; Nectin 1; Herpesvirus Entry Mediator C; Herpesvirus Ig-like receptor
Product Gene Name
PVRL1 elisa kit
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sample Manual Insert
Download Sample PDF Manual View Sample PDF Manual
Request for Current Manual Insert
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3D Structure
ModBase 3D Structure for Q15223
Specificity
This assay has high sensitivity and excellent specificity for detection of Poliovirus Receptor Related Protein 1 (PVRL1).
No significant cross-reactivity or interference between Poliovirus Receptor Related Protein 1 (PVRL1) and analogues was observed.
Assay Type
Double-antibody Sandwich
Samples
Tissue Homogenates, Cell Lysates and Other Biological Fluids
Detection Range
0.156-10ng/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Poliovirus Receptor Related Protein 1 (PVRL1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Poliovirus Receptor Related Protein 1 (PVRL1) were tested on 3 different plates, 8 replicates in each plate.
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customera??s specifications, please inquire.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of PVRL1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for PVRL1 purchase
MBS2703330 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Poliovirus Receptor Related Protein 1 (PVRL1) ELISA Kit target analytes in
biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PVRL1. The ELISA analytical biochemical technique of the MBS2703330 kit is based on PVRL1 antibody-PVRL1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PVRL1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PVRL1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
PVRL1 elisa kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Poliovirus Receptor Related Protein 1 (PVRL1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Poliovirus Receptor Related Protein 1 (PVRL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Poliovirus Receptor Related Protein 1 (PVRL1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Poliovirus Receptor Related Protein 1 (PVRL1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Typical Testing Data/Standard Curve (for reference only) of PVRL1 elisa kit
NCBI/Uniprot data below describe general gene information for PVRL1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_002846.3
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NCBI GenBank Nucleotide #
NM_002855.4
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UniProt Primary Accession #
Q15223
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UniProt Secondary Accession #
O75465; Q2M3D3; Q9HBE6; Q9HBW2[Other Products]
UniProt Related Accession #
Q15223[Other Products]
Molecular Weight
39,150 Da
NCBI Official Full Name
nectin-1 isoform 1
NCBI Official Synonym Full Names
nectin cell adhesion molecule 1
NCBI Official Symbol
NECTIN1??[Similar Products]
NCBI Official Synonym Symbols
ED4; PRR; HIgR; HV1S; HVEC; OFC7; PRR1; PVRR; CD111; PVRL1; PVRR1; SK-12; CLPED1; nectin-1
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NCBI Protein Information
nectin-1
UniProt Protein Name
Nectin-1
UniProt Synonym Protein Names
Herpes virus entry mediator C; Herpesvirus entry mediator C; HveC; Herpesvirus Ig-like receptor; HIgR; Nectin cell adhesion molecule 1
UniProt Gene Name
NECTIN1??[Similar Products]
UniProt Synonym Gene Names
Herpesvirus entry mediator C; HveC; HIgR??[Similar Products]
NCBI Summary for PVRL1
This gene encodes an adhesion protein that plays a role in the organization of adherens junctions and tight junctions in epithelial and endothelial cells. The protein is a calcium(2+)-independent cell-cell adhesion molecule that belongs to the immunoglobulin superfamily and has 3 extracellular immunoglobulin-like loops, a single transmembrane domain (in some isoforms), and a cytoplasmic region. This protein acts as a receptor for glycoprotein D (gD) of herpes simplex viruses 1 and 2 (HSV-1, HSV-2), and pseudorabies virus (PRV) and mediates viral entry into epithelial and neuronal cells. Mutations in this gene cause cleft lip and palate/ectodermal dysplasia 1 syndrome (CLPED1) as well as non-syndromic cleft lip with or without cleft palate (CL/P). Alternative splicing results in multiple transcript variants encoding proteins with distinct C-termini. [provided by RefSeq, Oct 2009]
UniProt Comments for PVRL1
Promotes cell-cell contacts by forming homophilic or heterophilic trans-dimers. Heterophilic interactions have been detected between NECTIN1 and NECTIN3 and between NECTIN1 and NECTIN4. Has some neurite outgrowth-promoting activity.
Research Articles on PVRL1
1. Pseudorabies virus infects host cells via both human and swine nectin-1.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.