For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of Human PVRL1. No significant cross-reactivity or interference between Human PVRL1 and analogues was observed.

Store at 2-8 degree C.

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Small volumes of PVRL1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS283198 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Poliovirus receptor-related protein 1 (PVRL1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PVRL1. The ELISA analytical biochemical technique of the MBS283198 kit is based on PVRL1 antibody-PVRL1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PVRL1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PVRL1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate PVRL1 in samples. An antibody specific for PVRL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPVRL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PVRL1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PVRL1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

39,150 Da

nectin cell adhesion molecule 1

nectin-1

Nectin-1

Herpesvirus entry mediator C; HveC; HIgR??[Similar Products]

This gene encodes an adhesion protein that plays a role in the organization of adherens junctions and tight junctions in epithelial and endothelial cells. The protein is a calcium(2+)-independent cell-cell adhesion molecule that belongs to the immunoglobulin superfamily and has 3 extracellular immunoglobulin-like loops, a single transmembrane domain (in some isoforms), and a cytoplasmic region. This protein acts as a receptor for glycoprotein D (gD) of herpes simplex viruses 1 and 2 (HSV-1, HSV-2), and pseudorabies virus (PRV) and mediates viral entry into epithelial and neuronal cells. Mutations in this gene cause cleft lip and palate/ectodermal dysplasia 1 syndrome (CLPED1) as well as non-syndromic cleft lip with or without cleft palate (CL/P). Alternative splicing results in multiple transcript variants encoding proteins with distinct C-termini. [provided by RefSeq, Oct 2009]

nectin 1: Promotes cell-cell contacts by forming homophilic or heterophilic trans-dimers. Heterophilic interactions have been detected between PVRL1/nectin-1 and PVRL3/nectin-3 and between PVRL1/nectin-1 and PVRL4/nectin-4. Defects in PVRL1 are the cause of ectodermal dysplasia Margarita Island type (EDMI); also known as Zlotogora-Ogur syndrome, cleft lip/palate-ectodermal dysplasia syndrome (CLPED1) or ectodermal dysplasia 4. Ectodermal dysplasia defines a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. EDMI is an autosomal recessive syndrome characterized by the association of cleft lip/palate, ectodermal dysplasia (sparse short and dry scalp hair, sparse eyebrows and eyelashes), and partial syndactyly of the fingers and/or toes. Two thirds of the patients do not manifest oral cleft but present with abnormal teeth and nails. Defects in PVRL1 are the cause of non-syndromic orofacial cleft type 7 (OFC7). Non-syndromic orofacial cleft is a common birth defect consisting of cleft lips with or without cleft palate. Cleft lips are associated with cleft palate in two- third of cases. A cleft lip can occur on one or both sides and range in severity from a simple notch in the upper lip to a complete opening in the lip extending into the floor of the nostril and involving the upper gum. Belongs to the nectin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11q23.3

Cellular Component: adherens junction; apical junction complex; axon; catenin complex; cell-cell adherens junction; cell-cell contact zone; extracellular region; integral component of membrane; integral component of plasma membrane; intracellular membrane-bound organelle; membrane; plasma membrane; presynaptic membrane

Molecular Function: carbohydrate binding; cell adhesion molecule binding; coreceptor activity; identical protein binding; protein binding; protein heterodimerization activity; protein homodimerization activity; receptor binding; viral receptor activity; virion binding

Biological Process: axon guidance; cell adhesion; cell recognition; entry of virus into host cell; heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules; homophilic cell adhesion; immune response; iron ion transport; lens morphogenesis in camera-type eye; regulation of synapse assembly; retina development in camera-type eye; signal transduction

Disease: Cleft Lip/palate-ectodermal Dysplasia Syndrome

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