For Research Use Only. Not for use in diagnostic procedures.

Store all reagents at 2-8 degree C.

We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of SAR1B elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS7208963 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the GTP binding protein SAR1b (SAR1B) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SAR1B. The ELISA analytical biochemical technique of the MBS7208963 kit is based on SAR1B antibody-SAR1B antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SAR1B antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SAR1B. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Signal Transduction

22,410 Da

secretion associated, Ras related GTPase 1B

GTP-binding protein SAR1b; 2310075M17Rik; SAR1 homolog B; SAR1a gene homolog 2; GTP-binding protein B; GTP-binding protein Sara

GTP-binding protein SAR1b

SARA2; SARB; GTBPB??[Similar Products]

SAR1B_HUMAN

The protein encoded by this gene is a small GTPase that acts as a homodimer. The encoded protein is activated by the guanine nucleotide exchange factor PREB and is involved in protein transport from the endoplasmic reticulum to the Golgi. This protein is part of the COPII coat complex. Defects in this gene are a cause of chylomicron retention disease (CMRD), also known as Anderson disease (ANDD). Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Mar 2010]

SAR1B: Involved in transport from the endoplasmic reticulum to the Golgi apparatus. Activated by the guanine nucleotide exchange factor PREB. Involved in the selection of the protein cargo and the assembly of the COPII coat complex. Defects in SAR1B are the cause of chylomicron retention disease (CMRD); also known as Anderson disease (ANDD). CMRD is an autosomal recessive disorder of severe fat malabsorption associated with failure to thrive in infancy. The condition is characterized by deficiency of fat-soluble vitamins, low blood cholesterol levels, and a selective absence of chylomicrons from blood. Affected individuals accumulate chylomicron-like particles in membrane-bound compartments of enterocytes, which contain large cytosolic lipid droplets. Belongs to the small GTPase superfamily. SAR1 family.

Protein type: Motility/polarity/chemotaxis; G protein; G protein, monomeric; G protein, monomeric, SAR1

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: endoplasmic reticulum membrane; cytosol

Molecular Function: GTPase activity; GTP binding; metal ion binding

Biological Process: COPII coating of Golgi vesicle; intracellular protein transport; antigen processing and presentation of peptide antigen via MHC class I; ER to Golgi vesicle-mediated transport; cellular protein metabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class II; protein amino acid N-linked glycosylation via asparagine; post-translational protein modification

Disease: Chylomicron Retention Disease

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