For Research Use Only. Not for use in diagnostic procedures.

Chromosome: X; NC_000023.10 (15363713..15402535, complement). Location: Xp22.31

This assay has high sensitivity and excellent specificity for detection of VEGF-D. No significant cross-reactivity or interference between VEGF-D and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between VEGF-D and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C

We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of VEGF-D elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS742272 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Vascular Endothelial Growth Factor D (VEGF-D) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing VEGF-D. The ELISA analytical biochemical technique of the MBS742272 kit is based on VEGF-D antibody-VEGF-D antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect VEGF-D antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, VEGF-D. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Intended Uses: This VEGF-D ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Goat VEGF-D. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: VEGF-D ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-VEGF-D antibody and an VEGF-D-HRP conjugate. The assay sample and buffer are incubated together with VEGF-D-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the VEGF-D concentration since VEGF-D from samples and VEGF-D-HRP conjugate compete for the anti-VEGF-D antibody . Since the number of sites is limited, as more sites are occupied by VEGF-D from the sample, fewer sites are left to bind VEGF-D-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The VEGF-D concentration in each sample is interpolated from this standard curve.

Cardiovascular

40,444 Da

c-fos induced growth factor (vascular endothelial growth factor D)

vascular endothelial growth factor D

Vascular endothelial growth factor D

VEGFD; VEGF-D; FIGF??[Similar Products]

VEGFD_HUMAN

The protein encoded by this gene is a member of the platelet-derived growth factor/vascular endothelial growth factor (PDGF/VEGF) family and is active in angiogenesis, lymphangiogenesis, and endothelial cell growth. This secreted protein undergoes a complex proteolytic maturation, generating multiple processed forms which bind and activate VEGFR-2 and VEGFR-3 receptors. This protein is structurally and functionally similar to vascular endothelial growth factor C. Read-through transcription has been observed between this locus and the upstream PIR (GeneID 8544) locus. [provided by RefSeq, Feb 2011]

VEGFD: Growth factor active in angiogenesis, lymphangiogenesis and endothelial cell growth, stimulating their proliferation and migration and also has effects on the permeability of blood vessels. May function in the formation of the venous and lymphatic vascular systems during embryogenesis, and also in the maintenance of differentiated lymphatic endothelium in *****s. Binds and activates VEGFR-2 (KDR/FLK1) and VEGFR-3 (FLT4) receptors. Belongs to the PDGF/VEGF growth factor family.

Protein type: Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide; Cytokine

Chromosomal Location of Human Ortholog: Xp22.31

Cellular Component: extracellular space; membrane; extracellular region

Molecular Function: protein homodimerization activity; growth factor activity; platelet-derived growth factor receptor binding; vascular endothelial growth factor receptor 3 binding; vascular endothelial growth factor receptor binding; chemoattractant activity

Biological Process: cell proliferation; platelet activation; platelet degranulation; positive chemotaxis; positive regulation of cell division; positive regulation of cell proliferation; angiogenesis; cell differentiation; vascular endothelial growth factor receptor signaling pathway; blood coagulation; induction of positive chemotaxis

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