Full Product Name
Anti-AGTR1 Rabbit Monoclonal Antibody
Product Synonym Names
Type-1 angiotensin II receptor; AT1AR; AT1BR; Angiotensin II type-1 receptor; AT1; AGTR1; AGTR1A; AGTR1B; AT2R1; AT2R1B
Product Gene Name
anti-AGTR1 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P30556
Species Reactivity
Human, Mouse, Rat
Purity/Purification
Affinity-chromatography
Immunogen
A synthesized peptide derived from human AGTR1
Subcellular Localization
Cell membrane; Multi-pass membrane protein.
Tissue Specificity
Liver, lung, adrenal and adrenocortical adenomas.
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Other Notes
Small volumes of anti-AGTR1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-AGTR1 antibody
Receptor for angiotensin II. Mediates its action by association with G proteins that activate a phosphatidylinositol- calcium second messenger system.
Product Categories/Family for anti-AGTR1 antibody
Cancer; Invasion/Microenvironment; Angiogenesis; Angiogenic Growth Factors; Cardiovascular; Blood; Blood Pressure Regulation; Cancer; Cancer Metabolism; Cellular Metabolic Process; Metabolism; Types of Disease
Applications Tested/Suitable for anti-AGTR1 antibody
Western Blot (WB)
Application Notes for anti-AGTR1 antibody
WB: 1:500-1:2000
Western Blot (WB) of anti-AGTR1 antibody
Western blot analysis of AGTR1 expression in (1) HeLa cell lysate; (2) PC-12 cell lysate (MBS179592).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AGTR1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for AGTR1
NCBI/Uniprot data below describe general gene information for AGTR1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000676.1
[Other Products]
NCBI GenBank Nucleotide #
NM_000685.4
[Other Products]
UniProt Primary Accession #
P30556
[Other Products]
UniProt Secondary Accession #
Q13725; Q8TBK4[Other Products]
Molecular Weight
41061 MW
NCBI Official Full Name
type-1 angiotensin II receptor isoform 1
UniProt Protein Name
Type-1 angiotensin II receptor
UniProt Synonym Protein Names
AT1AR; AT1BR; Angiotensin II type-1 receptor; AT1
Protein Family
Type-1 angiotensin II receptor
UniProt Gene Name
AGTR1??[Similar Products]
UniProt Synonym Gene Names
AGTR1A; AGTR1B; AT2R1; AT2R1B; AT1??[Similar Products]
UniProt Comments for AGTR1
Receptor for angiotensin II. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
