For Research Use Only. Not for use in diagnostic procedures.

Store all reagents at 2-8 degree C.

We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of AT2R1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS742321 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Angiotensin 2 Receptor 1 Antibody (AT2R1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing AT2R1. The ELISA analytical biochemical technique of the MBS742321 kit is based on AT2R1 antibody-AT2R1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect AT2R1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, AT2R1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the Assay: AT1R-AA ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-AT1R-AA antibody and an AT1R-AA-HRP conjugate. The assay sample and buffer are incubated together with AT1R-AA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AT1R-AA concentration since AT1R-AA from samples and AT1R-AA-HRP conjugate compete for the anti-AT1R-AA antibody . Since the number of sites is limited, as more sites are occupied by AT1R-AA from the sample, fewer sites are left to bind AT1R-AA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AT1R-AA concentration in each sample is interpolated from this standard curve.

Signal Transduction

41,061 Da

angiotensin II receptor, type 1

type-1 angiotensin II receptor; type-1B angiotensin II receptor

Type-1 angiotensin II receptor

AGTR1A; AGTR1B; AT2R1; AT2R1B; AT1??[Similar Products]

AGTR1_HUMAN

Angiotensin II is a potent vasopressor hormone and a primary regulator of aldosterone secretion. It is an important effector controlling blood pressure and volume in the cardiovascular system. It acts through at least two types of receptors. This gene encodes the type 1 receptor which is thought to mediate the major cardiovascular effects of angiotensin II. This gene may play a role in the generation of reperfusion arrhythmias following restoration of blood flow to ischemic or infarcted myocardium. It was previously thought that a related gene, denoted as AGTR1B, existed; however, it is now believed that there is only one type 1 receptor gene in humans. Multiple alternatively spliced transcript variants have been reported for this gene. [provided by RefSeq, Jul 2012]

AT1: receptor for angiotensin II. Angiotensin II is a potent vasopressor hormone and a primary regulator of aldosterone secretion. It is an important effector controlling blood pressure and volume in the cardiovascular system. Mediates the major cardiovascular effects of angiotensin II. May play role in the generation of reperfusion arrhythmias following restoration of blood flow to ischemic or infarcted myocardium. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; GPCR, family 1; Receptor, GPCR

Chromosomal Location of Human Ortholog: 3q24

Cellular Component: integral to plasma membrane; plasma membrane; integral to membrane

Molecular Function: protein binding; angiotensin type II receptor activity; protein heterodimerization activity; angiotensin type I receptor activity; bradykinin receptor binding

Biological Process: elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); regulation of vasodilation; renin-angiotensin regulation of aldosterone production; positive regulation of cellular protein metabolic process; calcium-mediated signaling; regulation of systemic arterial blood pressure by renin-angiotensin; Rho protein signal transduction; regulation of cell proliferation; G-protein coupled receptor protein signaling pathway; elevation of cytosolic calcium ion concentration; renin-angiotensin regulation of blood vessel size; regulation of vasoconstriction; regulation of inflammatory response; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); regulation of cell growth; kidney development; positive regulation of phospholipase A2 activity; positive regulation of inflammatory response; positive regulation of NAD(P)H oxidase activity

Disease: Renal Tubular Dysgenesis; Hypertension, Essential

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