Product Name
TALDO1, siRNA
Full Product Name
TALDO1 siRNA (Mouse)
Product Synonym Names
TAL; TALDO; Transaldolase
Product Gene Name
TALDO1 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q93092
Specificity
TALDO1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse TALDO1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of TALDO1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
TALDO1 sirna
siRNA to inhibit TALDO1 expression using RNA interference
Applications Tested/Suitable for TALDO1 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for TALDO1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_035658.1
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NCBI GenBank Nucleotide #
NM_011528.4
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UniProt Primary Accession #
Q93092
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UniProt Secondary Accession #
P70358; P70703; Q52KM4[Other Products]
UniProt Related Accession #
Q93092[Other Products]
Molecular Weight
37,387 Da
NCBI Official Full Name
transaldolase
NCBI Official Synonym Full Names
transaldolase 1
NCBI Official Symbol
Taldo1??[Similar Products]
NCBI Protein Information
transaldolase
UniProt Protein Name
Transaldolase
Protein Family
Transaldolase
UniProt Gene Name
Taldo1??[Similar Products]
UniProt Synonym Gene Names
Tal; Taldo??[Similar Products]
UniProt Entry Name
TALDO_MOUSE
NCBI Summary for TALDO1
This gene encodes a key enzyme of the nonoxidative pentose phosphate pathway that provides ribose-5-phosphate for nucleic acid synthesis and nicotinamide adenine dinucleotide phosphate (NADPH) for lipid biosynthesis. The encoded protein is important for maintaining structure and function of mitochondria. Studies in knockout mice identify that deficiency of this gene product is a cause of sperm dysmotility and male infertility. Deficiency of this protein has also been identified as a cause of hepatocarcinogenesis in mice. Two related pseudogenes have been identified on chromosome 10. [provided by RefSeq, Mar 2010]
UniProt Comments for TALDO1
TALDO1: Transaldolase is important for the balance of metabolites in the pentose-phosphate pathway. Defects in TALDO1 are the cause of transaldolase 1 deficiency (TALDO1 deficiency). It results in telangiectases of the skin, hepatosplenomegaly, and enlarged clitoris. Belongs to the transaldolase family. Type 1 subfamily.
Protein type: Transferase; EC 2.2.1.2; Carbohydrate Metabolism - pentose phosphate pathway
Cellular Component: intracellular membrane-bound organelle; cytoplasm; nucleus; cytosol
Molecular Function: monosaccharide binding; transferase activity; transaldolase activity; carbohydrate binding; catalytic activity
Biological Process: pentose-phosphate shunt; carbohydrate metabolic process; pentose-phosphate shunt, non-oxidative branch; glyceraldehyde-3-phosphate metabolic process; fructose 6-phosphate metabolic process
Research Articles on TALDO1
1. Data show that erythronic acid was identified as a major abnormal metabolite in all patients and in knock-out TALDO mice implicating an as yet unknown biochemical pathway in this disease.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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