Full Product Name
EYS siRNA (Human)
Product Synonym Names
C6orf178; C6orf179; C6orf180; EGFL10; EGFL11; SPAM; Protein eyes shut homolog; Epidermal growth factor-like protein 10; EGF-like protein 10; Epidermal growth factor-like protein 11; EGF-like protein 11; Protein spacemaker homolog
Product Gene Name
EYS sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q5T1H1
Specificity
EYS siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human EYS gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of EYS sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
EYS sirna
siRNA to inhibit EYS expression using RNA interference
Applications Tested/Suitable for EYS sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for EYS. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001136272.1
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NCBI GenBank Nucleotide #
NM_001142800.1
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UniProt Primary Accession #
Q5T1H1
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UniProt Secondary Accession #
Q5SZM4; Q5T3C8; Q5T669; A2RUR2; A8MVE7; B7TYK8; B7UUQ3; B7ZBE7; B7ZBE8; B7ZBR3; B9ZVD2[Other Products]
UniProt Related Accession #
Q5T1H1[Other Products]
Molecular Weight
69,877 Da
NCBI Official Full Name
protein eyes shut homolog isoform 1
NCBI Official Synonym Full Names
eyes shut homolog (Drosophila)
NCBI Official Symbol
EYS??[Similar Products]
NCBI Official Synonym Symbols
RP25; SPAM; EGFL10; EGFL11; C6orf178; C6orf179; C6orf180; bA74E24.1; dJ22I17.2; bA166P24.2; bA307F22.3; dJ1018A4.2; dJ303F19.1
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NCBI Protein Information
protein eyes shut homolog
UniProt Protein Name
Protein eyes shut homolog
UniProt Synonym Protein Names
Epidermal growth factor-like protein 10; EGF-like protein 10; Epidermal growth factor-like protein 11; EGF-like protein 11; Protein spacemaker homolog
Protein Family
Protein eyes shut
UniProt Gene Name
EYS??[Similar Products]
UniProt Synonym Gene Names
C6orf178; C6orf179; C6orf180; EGFL10; EGFL11; SPAM; EGF-like protein 10; EGF-like protein 11??[Similar Products]
UniProt Entry Name
EYS_HUMAN
NCBI Summary for EYS
The product of this gene contains multiple epidermal growth factor (EGF)-like and LamG domains. The protein is expressed in the photoreceptor layer of the retina, and the gene is mutated in autosomal recessive retinitis pigmentosa. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2008]
UniProt Comments for EYS
EYS: Required to maintain the integrity of photoreceptor cells. Defects in EYS are the cause of retinitis pigmentosa type 25 (RP25). RP leads to degeneration of retinal photoreceptor cells. Patients suffer from night blindness, beginning at approximately 25 years of age, and deterioration of visual acuity (central vision), beginning at approximately 30 years of age. By age 55 to 60 years, many affected subjects have no perception of light in either eye. Belongs to the EYS family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 6q12
Molecular Function: calcium ion binding
Biological Process: detection of light stimulus involved in visual perception; skeletal muscle regeneration
Disease: Retinitis Pigmentosa 25
Research Articles on EYS
1. New variants were found to be located on the USH2A, RPGR, EYS, and RHO genes
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
