Full Product Name
FGB siRNA (Rat)
Product Synonym Names
Fibrinogen beta chain; Liver regeneration-related protein LRRG036/LRRG043/LRRG189
Product Gene Name
FGB sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P14480
Specificity
FGB siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat FGB gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of FGB sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
FGB sirna
siRNA to inhibit FGB expression using RNA interference
Applications Tested/Suitable for FGB sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for FGB. It may not necessarily be applicable to this product.
NCBI Accession #
NP_064456.2
[Other Products]
NCBI GenBank Nucleotide #
NM_020071.2
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UniProt Primary Accession #
P14480
[Other Products]
UniProt Secondary Accession #
Q5I0P7; Q7TME5[Other Products]
UniProt Related Accession #
P14480[Other Products]
Molecular Weight
56,613 Da
NCBI Official Full Name
fibrinogen beta chain
NCBI Official Synonym Full Names
fibrinogen beta chain
NCBI Official Symbol
Fgb??[Similar Products]
NCBI Official Synonym Symbols
Ab1-181; Ab1-216; Ac1-581
??[Similar Products]
NCBI Protein Information
fibrinogen beta chain
UniProt Protein Name
Fibrinogen beta chain
UniProt Synonym Protein Names
Liver regeneration-related protein LRRG036/LRRG043/LRRG189
Protein Family
Fibrinogen
UniProt Gene Name
Fgb??[Similar Products]
UniProt Entry Name
FIBB_RAT
NCBI Summary for FGB
cleaved by thrombin; plays a role in wound healing [RGD, Feb 2006]
UniProt Comments for FGB
FGB: Fibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation. Defects in FGB are a cause of congenital afibrinogenemia (CAFBN). This rare autosomal recessive disorder is characterized by bleeding that varies from mild to severe and by complete absence or extremely low levels of plasma and platelet fibrinogen. Patients with congenital fibrinogen abnormalities can manifest different clinical pictures. Some cases are clinically silent, some show a tendency toward bleeding and some show a predisposition for thrombosis with or without bleeding.
Protein type: Adaptor/scaffold; Cell surface; Secreted; Secreted, signal peptide
Cellular Component: extracellular space; cell surface; cytoplasm; fibrinogen complex; cell cortex; vesicle; external side of plasma membrane
Molecular Function: protein binding, bridging; chaperone binding; cell adhesion molecule binding; structural molecule activity; receptor binding
Biological Process: protein polymerization; cellular protein complex assembly; positive regulation of heterotypic cell-cell adhesion; positive regulation of protein secretion; cell-matrix adhesion; positive regulation of vasoconstriction; innate immune response; signal transduction; response to calcium ion; positive regulation of exocytosis
Research Articles on FGB
1. Plasma fibrinogen levels were significantly increased in the experimental periodontitis groups.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
