Product Name
COL5A1, siRNA
Full Product Name
COL5A1 siRNA (Human)
Product Synonym Names
Collagen alpha-1(V) chain
Product Gene Name
COL5A1 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P20908
Specificity
COL5A1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human COL5A1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of COL5A1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
COL5A1 sirna
siRNA to inhibit COL5A1 expression using RNA interference
Applications Tested/Suitable for COL5A1 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for COL5A1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000084.3
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NCBI GenBank Nucleotide #
NM_000093.4
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UniProt Primary Accession #
P20908
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UniProt Secondary Accession #
Q15094; Q5SUX4[Other Products]
UniProt Related Accession #
P20908[Other Products]
Molecular Weight
183,560 Da
NCBI Official Full Name
collagen alpha-1(V) chain isoform 1 preproprotein
NCBI Official Synonym Full Names
collagen, type V, alpha 1
NCBI Official Symbol
COL5A1??[Similar Products]
NCBI Official Synonym Symbols
EDSC
??[Similar Products]
NCBI Protein Information
collagen alpha-1(V) chain
UniProt Protein Name
Collagen alpha-1(V) chain
UniProt Gene Name
COL5A1??[Similar Products]
UniProt Entry Name
CO5A1_HUMAN
NCBI Summary for COL5A1
This gene encodes an alpha chain for one of the low abundance fibrillar collagens. Fibrillar collagen molecules are trimers that can be composed of one or more types of alpha chains. Type V collagen is found in tissues containing type I collagen and appears to regulate the assembly of heterotypic fibers composed of both type I and type V collagen. This gene product is closely related to type XI collagen and it is possible that the collagen chains of types V and XI constitute a single collagen type with tissue-specific chain combinations. The encoded procollagen protein occurs commonly as the heterotrimer pro-alpha1(V)-pro-alpha1(V)-pro-alpha2(V). Mutations in this gene are associated with Ehlers-Danlos syndrome, types I and II. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, May 2013]
UniProt Comments for COL5A1
COL5A1: Type V collagen is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V collagen binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin. Defects in COL5A1 are a cause of Ehlers-Danlos syndrome type 1 (EDS1); also known as Ehlers-Danlos syndrome gravis or severe classic type Ehlers-Danlos syndrome. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS1 is the severe form of classic Ehlers-Danlos syndrome. Defects in COL5A1 are a cause of Ehlers-Danlos syndrome type 2 (EDS2); also known as Ehlers-Danlos syndrome mitis or mild classic type Ehlers Danlos syndrome. Belongs to the fibrillar collagen family.
Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted; Extracellular matrix; Cell adhesion
Chromosomal Location of Human Ortholog: 9q34.2-q34.3
Cellular Component: extracellular matrix; collagen type V; endoplasmic reticulum lumen; extracellular region; basement membrane
Molecular Function: integrin binding; heparin binding; proteoglycan binding; extracellular matrix structural constituent; metal ion binding; platelet-derived growth factor binding
Biological Process: skin development; heart morphogenesis; axon guidance; blood vessel development; cell migration; extracellular matrix organization and biogenesis; collagen fibril organization; collagen catabolic process; extracellular matrix disassembly; integrin biosynthetic process; regulation of cellular component organization and biogenesis; fibril organization and biogenesis; cell adhesion; collagen biosynthetic process; eye morphogenesis; wound healing, spreading of epidermal cells
Disease: Ehlers-danlos Syndrome, Type I
Research Articles on COL5A1
1. Running economy, the energy cost or oxygen uptake for a given submaximal running speed, was not associated with variants within the COL5A1gene.
Precautions
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Disclaimer
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