Collagen alpha-1(V) chain; COL5A1

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit Ig

Rabbit

This COL5A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 54-82 amino acids from the N-terminal region of human COL5A1.

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Vial Concentration: 0.48 (lot specific)

Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Small volumes of anti-COL5A1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

COL5A1 encodes an alpha chain for one of the low
abundance fibrillar collagens. Fibrillar collagen molecules are
trimers that can be composed of one or more types of alpha chains.
Type V collagen is found in tissues containing type I collagen and
appears to regulate the assembly of heterotypic fibers composed of
both type I and type V collagen. COL5A1 product is closely
related to type XI collagen and it is possible that the collagen
chains of types V and XI constitute a single collagen type with
tissue-specific chain combinations. Mutations in this gene are
associated with Ehlers-Danlos syndrome, types I and II. [provided
by RefSeq].

Signal Transduction

ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)

WB~~1:1000

COL5A1 Antibody (N-term) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the COL5A1 antibody detected the COL5A1 protein (arrow).

COL5A1 Antibody (N-term) western blot analysis in mouse bladder tissue lysates (35ug/lane).This demonstrates the COL5A1 antibody detected the COL5A1 protein (arrow).

COL5A1 antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the COL5A1 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

183560

collagen, type V, alpha 1

collagen alpha-1(V) chain

Collagen alpha-1(V) chain

CO5A1_HUMAN

This gene encodes an alpha chain for one of the low abundance fibrillar collagens. Fibrillar collagen molecules are trimers that can be composed of one or more types of alpha chains. Type V collagen is found in tissues containing type I collagen and appears to regulate the assembly of heterotypic fibers composed of both type I and type V collagen. This gene product is closely related to type XI collagen and it is possible that the collagen chains of types V and XI constitute a single collagen type with tissue-specific chain combinations. The encoded procollagen protein occurs commonly as the heterotrimer pro-alpha1(V)-pro-alpha1(V)-pro-alpha2(V). Mutations in this gene are associated with Ehlers-Danlos syndrome, types I and II. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, May 2013]

COL5A1: Type V collagen is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V collagen binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin. Defects in COL5A1 are a cause of Ehlers-Danlos syndrome type 1 (EDS1); also known as Ehlers-Danlos syndrome gravis or severe classic type Ehlers-Danlos syndrome. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS1 is the severe form of classic Ehlers-Danlos syndrome. Defects in COL5A1 are a cause of Ehlers-Danlos syndrome type 2 (EDS2); also known as Ehlers-Danlos syndrome mitis or mild classic type Ehlers Danlos syndrome. Belongs to the fibrillar collagen family.

Protein type: Secreted, signal peptide; Secreted; Extracellular matrix; Cell adhesion; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 9q34.2-q34.3

Cellular Component: extracellular matrix; collagen type V; endoplasmic reticulum lumen; extracellular region; basement membrane

Molecular Function: integrin binding; heparin binding; proteoglycan binding; platelet-derived growth factor binding; extracellular matrix structural constituent; metal ion binding

Biological Process: skin development; heart morphogenesis; blood vessel development; axon guidance; cell migration; extracellular matrix organization and biogenesis; collagen fibril organization; extracellular matrix disassembly; collagen catabolic process; integrin biosynthetic process; regulation of cellular component organization and biogenesis; fibril organization and biogenesis; cell adhesion; collagen biosynthetic process; eye morphogenesis; wound healing, spreading of epidermal cells

Disease: Ehlers-danlos Syndrome, Type I

Vitart, V., et al. Hum. Mol. Genet. 19(21):4304-4311(2010)
Romero, R., et al. Am. J. Obstet. Gynecol. 203 (4), 361 (2010) :
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Posthumus, M., et al. Med Sci Sports Exerc (2010) In press :
Borck, G., et al. Am. J. Med. Genet. A 152A (8), 2090-2093 (2010) :

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