Anti -COL5A2, NT (Collagen alpha-2(V) Chain)

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 2; NC_000002.11 (189896641..190044668, complement). Location: 2q14-q32

Polyclonal

IgG

Rabbit

Recognizes human COL5A2.

Affinity Purified
Purified by immunoaffinity chromatography.

Supplied as a liquid in PBS (without Mg2+, Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Small volumes of anti-COL5A2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Type V collagen is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V collagen binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin. Type V collagen is a key determinant in the assembly of tissue-specific matrices.

Antibodies; Abs to Collagen

ELISA (EL/EIA), Immunohistochemistry (IHC), Immunofluorescence (IF)

Suitable for use in ELISA, Immunofluorescence and Immunohistochemistry.
Dilution: ELISA: 1:40,000
Immunofluorescence: 1:100-1:500
Immunohistochemistry (Formalin fixed paraffin embedded): 10ug/ml

144,910 Da[Similar Products]

collagen, type V, alpha 2

collagen alpha-2(V) chain; collagen alpha-2(V) chain; AB collagen; type V preprocollagen alpha 2 chain; collagen, fetal membrane, A polypeptide

Collagen alpha-2(V) chain

CO5A2_HUMAN

This gene encodes an alpha chain for one of the low abundance fibrillar collagens. Fibrillar collagen molecules are trimers that can be composed of one or more types of alpha chains. Type V collagen is found in tissues containing type I collagen and appears to regulate the assembly of heterotypic fibers composed of both type I and type V collagen. This gene product is closely related to type XI collagen and it is possible that the collagen chains of types V and XI constitute a single collagen type with tissue-specific chain combinations. Mutations in this gene are associated with Ehlers-Danlos syndrome, types I and II. [provided by RefSeq, Jul 2008]

Function: Type V collagen is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V collagen binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin. Type V collagen is a key determinant in the assembly of tissue-specific matrices

By similarity.

Subunit structure: Trimers of two alpha 1(V) and one alpha 2(V) chains in most tissues and trimers of one alpha 1(V), one alpha 2(V), and one alpha 3(V) chains in placenta.

Subcellular location: Secreted ? extracellular space ? extracellular matrix

By similarity.

Domain: The C-terminal propeptide, also known as COLFI domain, have crucial roles in tissue growth and repair by controlling both the intracellular assembly of procollagen molecules and the extracellular assembly of collagen fibrils. It binds a calcium ion which is essential for its function

By similarity.

Post-translational modification: Prolines at the third position of the tripeptide repeating unit (G-X-P) are hydroxylated in some or all of the chains. Probably 3-hydroxylated on Pro-919 and Pro-1156 by LEPREL1.

Involvement in disease: Ehlers-Danlos syndrome 1 (EDS1) [MIM:130000]: A connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS1 is the severe form of classic Ehlers-Danlos syndrome.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.15Ehlers-Danlos syndrome 2 (EDS2) [MIM:130010]: Mild form of classic Ehlers-Danlos syndrome.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.17

Sequence similarities: Belongs to the fibrillar collagen family.Contains 1 fibrillar collagen NC1 domain.Contains 1 VWFC domain.

Sequence caution: The sequence AAH43613.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence AAY24185.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence BAD92282.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

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