For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

IgG

Rabbit

The antibody is a rabbit polyclonal antibody raised against MRE11A. It has beenselected for its ability to recognize MRE11A in immunohistochemical staining andwestern blotting.

Affinity Chromatography

Liquid

200ug/ml (lot specific)

Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.

Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customera??s specifications, please inquire.

Small volumes of anti-MRE11A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)

Western blotting: 0.2-2ug/mL;1:250-2500
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.

Western Blot: Sample: Human Serum; Primary Ab: 1ug/ml Rabbit Anti-Human MRE11A Antibody Second Ab: 0.2ug/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody

Western Blot: Sample: Recombinant protein.

DAB staining on fromalin fixed paraffin- embedded Kidney tissue)

81,070 Da

MRE11 homolog, double strand break repair nuclease

double-strand break repair protein MRE11; double-strand break repair protein MRE11A

Double-strand break repair protein MRE11

MRE11 homolog 1; MRE11 homolog A??[Similar Products]

This gene encodes a nuclear protein involved in homologous recombination, telomere length maintenance, and DNA double-strand break repair. By itself, the protein has 3' to 5' exonuclease activity and endonuclease activity. The protein forms a complex with the RAD50 homolog; this complex is required for nonhomologous joining of DNA ends and possesses increased single-stranded DNA endonuclease and 3' to 5' exonuclease activities. In conjunction with a DNA ligase, this protein promotes the joining of noncomplementary ends in vitro using short homologies near the ends of the DNA fragments. This gene has a pseudogene on chromosome 3. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

MRE11A: a nuclear protein involved in homologous recombination, telomere length maintenance, and DNA double-strand break repair. By itself, the protein has 3' to 5' exonuclease activity and endonuclease activity. The protein forms a complex with the RAD50 homolog; this complex is required for nonhomologous joining of DNA ends and possesses increased single-stranded DNA endonuclease and 3' to 5' exonuclease activities. In conjunction with a DNA ligase, this protein promotes the joining of noncomplementary ends in vitro using short homologies near the ends of the DNA fragments. Alternative splicing results in two different isoforms.

Protein type: Cell cycle regulation; DNA repair, damage; DNA-binding; Deoxyribonuclease

Chromosomal Location of Human Ortholog: 11q21

Cellular Component: chromosome, telomeric region; cytoplasm; cytosol; Mre11 complex; nuclear chromosome, telomeric region; nucleoplasm; nucleus; PML body

Molecular Function: 3'-5' exonuclease activity; ATP-dependent DNA helicase activity; cadherin binding; DNA binding; double-stranded DNA binding; endodeoxyribonuclease activity; identical protein binding; manganese ion binding; nuclease activity; protein binding; protein C-terminus binding; single-stranded DNA endodeoxyribonuclease activity

Biological Process: cell proliferation; cellular response to DNA damage stimulus; DNA double-strand break processing; DNA duplex unwinding; DNA recombination; DNA repair; DNA replication; DNA synthesis involved in DNA repair; double-strand break repair; double-strand break repair via homologous recombination; double-strand break repair via nonhomologous end joining; intra-S DNA damage checkpoint; mitotic cell cycle G2/M transition DNA damage checkpoint; negative regulation of apoptosis; negative regulation of DNA endoreduplication; nucleic acid phosphodiester bond hydrolysis; positive regulation of interferon type I production; positive regulation of kinase activity; positive regulation of protein amino acid autophosphorylation; positive regulation of telomere maintenance; reciprocal meiotic recombination; regulation of mitotic recombination; sister chromatid cohesion; strand displacement; synapsis; telomere maintenance via telomerase; telomeric 3' overhang formation; viral process

Disease: Ataxia-telangiectasia-like Disorder 1

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