Full Product Name
ELANE Antibody
Product Synonym Names
GE; NE; HLE; HNE; ELA2; SCN1; PMN-E
Product Gene Name
anti-ELANE antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P08246
Specificity
The antibody detects endogenous levels of total ELANE protein.
Purity/Purification
Antigen affinity purification
Form/Format
In pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
Concentration
0.9mg/ml (lot specific)
Immunogen
Synthetic peptide of human ELANE
Preparation and Storage
Store at -20 degree C.
Other Notes
Small volumes of anti-ELANE antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-ELANE antibody
Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode structurally similar proteins. The encoded preproprotein is proteolytically processed to generate the active protease. Following activation, this protease hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix. The enzyme may play a role in degenerative and inflammatory diseases through proteolysis of collagen-IV and elastin. This protein also degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN). This gene is present in a gene cluster on chromosome 19.
Product Categories/Family for anti-ELANE antibody
Total protein Ab
Applications Tested/Suitable for anti-ELANE antibody
Immunohistochemistry (IHC)
Application Notes for anti-ELANE antibody
IF: 1: 20-100
SDS-Page of anti-ELANE antibody
Gel: 6%SDS-PAGE Lysate: 40 μg, Lane 1-2: Hela and Raji cell lysates Primary antibody:DDHD1 Antibody at dilution 1/250 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 90 seconds
NCBI/Uniprot data below describe general gene information for ELANE. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001963
[Other Products]
NCBI GenBank Nucleotide #
NM_001972.3
[Other Products]
UniProt Primary Accession #
P08246
[Other Products]
UniProt Secondary Accession #
P09649; Q6B0D9; Q6LDP5[Other Products]
UniProt Related Accession #
P08246[Other Products]
Molecular Weight
28,518 Da
NCBI Official Full Name
neutrophil elastase preproprotein
NCBI Official Synonym Full Names
elastase, neutrophil expressed
NCBI Official Symbol
ELANE??[Similar Products]
NCBI Official Synonym Symbols
GE; NE; HLE; HNE; ELA2; SCN1; PMN-E
??[Similar Products]
NCBI Protein Information
neutrophil elastase
UniProt Protein Name
Neutrophil elastase
UniProt Synonym Protein Names
Bone marrow serine protease; Elastase-2; Human leukocyte elastase; HLE; Medullasin; PMN elastase
Protein Family
Neutrophil elastase
UniProt Gene Name
ELANE??[Similar Products]
UniProt Synonym Gene Names
ELA2; HLE??[Similar Products]
NCBI Summary for ELANE
Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode structurally similar proteins. The encoded preproprotein is proteolytically processed to generate the active protease. Following activation, this protease hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix. The enzyme may play a role in degenerative and inflammatory diseases through proteolysis of collagen-IV and elastin. This protein also degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN). This gene is present in a gene cluster on chromosome 19. [provided by RefSeq, Jan 2016]
UniProt Comments for ELANE
Modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis.
Research Articles on ELANE
1. Using different blood leukocyte and skin resident cell preparations, and recombinant proteins, the authors have identified that neutrophil elastase, but not other neutrophil derived proteases, cleaves IL-36Ra into its highly active antagonistic form.
Precautions
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Disclaimer
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