Product Name
ELANE, siRNA
Full Product Name
ELANE siRNA (Mouse)
Product Synonym Names
ELA2; Neutrophil elastase; Elastase-2; Leukocyte elastase
Product Gene Name
ELANE sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q3UP87
Specificity
ELANE siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse ELANE gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of ELANE sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
ELANE sirna
siRNA to inhibit ELANE expression using RNA interference
Applications Tested/Suitable for ELANE sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for ELANE. It may not necessarily be applicable to this product.
NCBI Accession #
NP_056594.2
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NCBI GenBank Nucleotide #
NM_015779.2
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UniProt Primary Accession #
Q3UP87
[Other Products]
UniProt Secondary Accession #
Q61515; A6H698[Other Products]
UniProt Related Accession #
Q3UP87[Other Products]
Molecular Weight
28,648 Da
NCBI Official Full Name
neutrophil elastase
NCBI Official Synonym Full Names
elastase, neutrophil expressed
NCBI Official Symbol
Elane??[Similar Products]
NCBI Official Synonym Symbols
NE; Ela2; F430011M15Rik
??[Similar Products]
NCBI Protein Information
neutrophil elastase
UniProt Protein Name
Neutrophil elastase
UniProt Synonym Protein Names
Elastase-2; Leukocyte elastase
Protein Family
Neutrophil elastase
UniProt Gene Name
Elane??[Similar Products]
UniProt Synonym Gene Names
Ela2??[Similar Products]
UniProt Entry Name
ELNE_MOUSE
UniProt Comments for ELANE
ELANE: Modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis. Defects in ELANE are a cause of cyclic haematopoiesis (CH); also known as cyclic neutropenia. CH is an autosomal dominant disease in which blood-cell production from the bone marrow oscillates with 21-day periodicity. Circulating neutrophils vary between almost normal numbers and zero. During intervals of neutropenia, affected individuals are at risk for opportunistic infection. Monocytes, platelets, lymphocytes and reticulocytes also cycle with the same frequency. Defects in ELANE are the cause of neutropenia severe congenital autosomal dominant type 1 (SCN1). SCN1 is a disorder of hematopoiesis characterized by a maturation arrest of granulopoiesis at the level of promyelocytes with peripheral blood absolute neutrophil counts below 0.5 x 10(9)/l and early onset of severe bacterial infections. Belongs to the peptidase S1 family. Elastase subfamily.
Protein type: Cell cycle regulation; Motility/polarity/chemotaxis; Cell surface; EC 3.4.21.37; Protease
Cellular Component: cell surface; transcriptional repressor complex; cytoplasm; secretory granule
Molecular Function: peptidase activity; heparin binding; protease binding; hydrolase activity; serine-type peptidase activity; cytokine binding; serine-type endopeptidase activity; endopeptidase activity; catalytic activity
Biological Process: transcription from RNA polymerase II promoter; positive regulation of immune response; positive regulation of smooth muscle cell proliferation; negative regulation of interleukin-8 biosynthetic process; positive regulation of interleukin-8 biosynthetic process; response to lipopolysaccharide; negative regulation of transcription from RNA polymerase II promoter; proteolysis; phagocytosis; defense response to bacterium; response to yeast; defense response to fungus; leukocyte migration; acute inflammatory response to antigenic stimulus; negative regulation of chemokine biosynthetic process; response to UV
Research Articles on ELANE
1. SerpinA3N attenuates neuropathic pain by inhibiting T cell-derived leukocyte elastase
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
