Collagen alpha-1(V) chain

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit

Recognizes endogenous levels of Collagen 5 alpha 1 protein.

The antibody was purified by immunogen affinity chromatography.

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.

Small volumes of anti-COL5A1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Rabbit polyclonal antibody to Collagen 5 alpha 1

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP)

WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/ICC (1/100 - 1/500), IP (1/10 - 1/100)

Western blot analysis of Collagen 5 alpha 1 expression in HeLa (A) whole cell lysates.

Immunohistochemical analysis of Collagen 5 alpha 1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of Collagen 5 alpha 1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

183,560 Da

collagen, type V, alpha 1

collagen alpha-1(V) chain; collagen alpha-1(V) chain

Collagen alpha-1(V) chain

CO5A1_HUMAN

This gene encodes an alpha chain for one of the low abundance fibrillar collagens. Fibrillar collagen molecules are trimers that can be composed of one or more types of alpha chains. Type V collagen is found in tissues containing type I collagen and appears to regulate the assembly of heterotypic fibers composed of both type I and type V collagen. This gene product is closely related to type XI collagen and it is possible that the collagen chains of types V and XI constitute a single collagen type with tissue-specific chain combinations. The encoded procollagen protein occurs commonly as the heterotrimer pro-alpha1(V)-pro-alpha1(V)-pro-alpha2(V). Mutations in this gene are associated with Ehlers-Danlos syndrome, types I and II. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, May 2013]

COL5A1: Type V collagen is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V collagen binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin. Defects in COL5A1 are a cause of Ehlers-Danlos syndrome type 1 (EDS1); also known as Ehlers-Danlos syndrome gravis or severe classic type Ehlers-Danlos syndrome. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS1 is the severe form of classic Ehlers-Danlos syndrome. Defects in COL5A1 are a cause of Ehlers-Danlos syndrome type 2 (EDS2); also known as Ehlers-Danlos syndrome mitis or mild classic type Ehlers Danlos syndrome. Belongs to the fibrillar collagen family.

Protein type: Cell adhesion; Secreted, signal peptide; Extracellular matrix; Secreted; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 9q34.2-q34.3

Cellular Component: extracellular matrix; collagen type V; endoplasmic reticulum lumen; extracellular region; basement membrane

Molecular Function: integrin binding; heparin binding; proteoglycan binding; extracellular matrix structural constituent; platelet-derived growth factor binding; metal ion binding

Biological Process: skin development; heart morphogenesis; blood vessel development; axon guidance; extracellular matrix organization and biogenesis; cell migration; collagen fibril organization; collagen catabolic process; extracellular matrix disassembly; integrin biosynthetic process; regulation of cellular component organization and biogenesis; fibril organization and biogenesis; cell adhesion; collagen biosynthetic process; wound healing, spreading of epidermal cells; eye morphogenesis

Disease: Ehlers-danlos Syndrome, Type I

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