Human Double-strand break repair protein MRE11A (MRE11A) ELISA kit; ATLD; HNGS1; MRE11; MRE11B; AT-like disease; DNA recombination and repair protein; double-strand break repair protein MRE11A; endo/exonuclease Mre11; meiotic recombination 11 homolog A; MRE11 meiotic recombination 11 homolog A (S. cerevisiae)

For Research Use Only. Not for use in diagnostic procedures.

No significant cross-reactivity or interference between Human MRE11A and analogues was observed.

Store all reagents at 2-8 degree C

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Small volumes of MRE11A elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS9336466 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the MRE11 meiotic recombination 11 homolog A (S. cerevisiae) (MRE11A) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MRE11A. The ELISA analytical biochemical technique of the MBS9336466 kit is based on MRE11A antibody-MRE11A antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MRE11A antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MRE11A. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated MRE11A concentrations in Human serum, plasma and other body fluids. Using Purified Human MRE11A antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add MRE11A and MRE11A antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of MRE11A in the samples is then determined by comparing the O.D. of the samples to the standard curve.

80,593 Da

MRE11 meiotic recombination 11 homolog A (S. cerevisiae)

double-strand break repair protein MRE11A; AT-like disease; MRE11 homolog 1; MRE11 homolog A; endo/exonuclease Mre11; meiotic recombination 11 homolog 1; meiotic recombination 11 homolog A; DNA recombination and repair protein

Double-strand break repair protein MRE11A

HNGS1; MRE11; MRE11 homolog 1; MRE11 homolog A??[Similar Products]

MRE11_HUMAN

This gene encodes a nuclear protein involved in homologous recombination, telomere length maintenance, and DNA double-strand break repair. By itself, the protein has 3' to 5' exonuclease activity and endonuclease activity. The protein forms a complex with the RAD50 homolog; this complex is required for nonhomologous joining of DNA ends and possesses increased single-stranded DNA endonuclease and 3' to 5' exonuclease activities. In conjunction with a DNA ligase, this protein promotes the joining of noncomplementary ends in vitro using short homologies near the ends of the DNA fragments. This gene has a pseudogene on chromosome 3. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

MRE11A: a nuclear protein involved in homologous recombination, telomere length maintenance, and DNA double-strand break repair. By itself, the protein has 3' to 5' exonuclease activity and endonuclease activity. The protein forms a complex with the RAD50 homolog; this complex is required for nonhomologous joining of DNA ends and possesses increased single-stranded DNA endonuclease and 3' to 5' exonuclease activities. In conjunction with a DNA ligase, this protein promotes the joining of noncomplementary ends in vitro using short homologies near the ends of the DNA fragments. Alternative splicing results in two different isoforms.

Protein type: DNA-binding; Cell cycle regulation; DNA repair, damage; Deoxyribonuclease

Chromosomal Location of Human Ortholog: 11q21

Cellular Component: nucleoplasm; chromosome, telomeric region; Mre11 complex; cytosol; chromatin; nucleus

Molecular Function: protein C-terminus binding; ATP-dependent DNA helicase activity; protein binding; nuclease activity; DNA binding; single-stranded DNA specific endodeoxyribonuclease activity; endonuclease activity; manganese ion binding; double-stranded DNA binding; 3'-5' exonuclease activity; endodeoxyribonuclease activity

Biological Process: positive regulation of kinase activity; sister chromatid cohesion; negative regulation of DNA endoreduplication; telomere maintenance via telomerase; DNA repair; regulation of mitotic recombination; positive regulation of protein amino acid autophosphorylation; DNA catabolic process, endonucleolytic; DNA duplex unwinding; double-strand break repair via nonhomologous end joining; double-strand break repair via homologous recombination; DNA recombination; cell proliferation; base-excision repair; intra-S DNA damage checkpoint; nucleotide-excision repair; mitotic cell cycle G2/M transition DNA damage checkpoint; double-strand break repair; meiotic recombination; synapsis; positive regulation of interferon type I production; innate immune response; response to DNA damage stimulus; telomere maintenance

Disease: Ataxia-telangiectasia-like Disorder 1

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