ELA-2; Elastase; HNE; NEG; Neutrophil Elastase (NEG), Human - >=96% (SDS-PAGE) - Lyophilized

For Research Use Only. Not for use in diagnostic procedures.

Human Neutrophils

>= 96% (SDS-PAGE). Multiple bands observed, identity as neutrophil elastase confirmed by Western Blot.

Powder lyophilized from sodium acetate, pH 5.5

At 2-8 degree C. Protect from moisture.
Handling: Handle as a potentially hazardous substance.

Small volumes of ELANE protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MyBioSource is the leading producer of human neutrophil Elastase (NEG) for clinical research, life science and diagnostic manufacturing uses.

Custom preparations, technical support, bulk quantities and aliquoting available.

Human Neutrophils, also known as neutrophilic polymorphonuclear leukocytes, are the major class of white blood cell in human peripheral blood. They, have a multilobed nucleus and neutrophilic granules, and are phagocytes and have an important role in engulfing and killing extracellular pathogens.

The neutrophil form of elastase is 218 amino acids long, with two asparagine-linked carbohydrate chains. Current studies show that both Cathepsin G and Neutrophil Elastase are key enzymes for tissue injury caused by activated neutrophils, such as occurs in Acute Lung injury.

Proteins; Antigens; Enzymes; Standards/controls; Native Proteins; Neutrophil Elastase (ela-2)

Western Blot (WB)

28,518 Da[Similar Products]

elastase, neutrophil expressed

neutrophil elastase; elastase-2; medullasin; PMN elastase; leukocyte elastase; elastase 2, neutrophil; human leukocyte elastase; polymorphonuclear elastase; bone marrow serine protease; granulocyte-derived elastase

Neutrophil elastase

ELA2; HLE??[Similar Products]

ELNE_HUMAN

Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins. The product of this gene hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix following the protein's release from activated neutrophils. The enzyme may play a role in degenerative and inflammatory diseases by its proteolysis of collagen-IV and elastin of the extracellular matrix. This protein degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN). This gene is clustered with other serine protease gene family members, azurocidin 1 and proteinase 3 genes, at chromosome 19pter. All 3 genes are expressed coordinately and their protein products are packaged together into azurophil granules during neutrophil differentiation. [provided by RefSeq, May 2009]

ELANE: Modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis. Defects in ELANE are a cause of cyclic haematopoiesis (CH); also known as cyclic neutropenia. CH is an autosomal dominant disease in which blood-cell production from the bone marrow oscillates with 21-day periodicity. Circulating neutrophils vary between almost normal numbers and zero. During intervals of neutropenia, affected individuals are at risk for opportunistic infection. Monocytes, platelets, lymphocytes and reticulocytes also cycle with the same frequency. Defects in ELANE are the cause of neutropenia severe congenital autosomal dominant type 1 (SCN1). SCN1 is a disorder of hematopoiesis characterized by a maturation arrest of granulopoiesis at the level of promyelocytes with peripheral blood absolute neutrophil counts below 0.5 x 10(9)/l and early onset of severe bacterial infections. Belongs to the peptidase S1 family. Elastase subfamily.

Protein type: Motility/polarity/chemotaxis; Cell cycle regulation; Cell surface; Protease; EC 3.4.21.37

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: cell surface; transcriptional repressor complex; cytoplasm; extracellular region; secretory granule

Molecular Function: heparin binding; peptidase activity; protein binding; protease binding; cytokine binding; serine-type endopeptidase activity; endopeptidase activity

Biological Process: extracellular matrix organization and biogenesis; positive regulation of immune response; positive regulation of smooth muscle cell proliferation; negative regulation of interleukin-8 biosynthetic process; positive regulation of interleukin-8 biosynthetic process; response to lipopolysaccharide; negative regulation of chemotaxis; negative regulation of transcription from RNA polymerase II promoter; proteolysis; phagocytosis; cellular calcium ion homeostasis; positive regulation of MAP kinase activity; extracellular matrix disassembly; collagen catabolic process; negative regulation of inflammatory response; defense response to bacterium; protein catabolic process; response to yeast; leukocyte migration; acute inflammatory response to antigenic stimulus; response to UV; negative regulation of chemokine biosynthetic process

Disease: Cyclic Neutropenia; Neutropenia, Severe Congenital, 1, Autosomal Dominant

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