Full Product Name
PHGDH Antibody Pair
Product Synonym Names
D-3-phosphoglycerate dehydrogenase; 3-PGDH; PGDH3; PHGDH
Product Gene Name
PHGDH antibody pair
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for O43175
Isotype
Capture: IgG
Detection: IgG
Host
Capture: Rabbit
Detection: Rabbit
Species Reactivity
Bovine, Pig, Dog, Chicken
Concentration
The concentrations and volumes of the antibodies may vary and be lot specific. Details will be on the vial labels. (lot specific)
Conjugation
Capture: Non-conjugated; Detection: Biotin conjugated
Recommended Standard
MOLT-4 cell; KM-12 cell; Hela cell
(Standards are recommendations only. Compatibility of the standards in the user developed application needs to be established and confirmed by the researcher.
Storage Buffer
Capture: 50% Glycerol, 0.01M PBS, PH 7.4
Detection: 50% Glycerol, 0.01M PBS, PH 7.4
Other Note
Immunogen information is company proprietary information. Antibodies are sold as a pair and not available separately.
Preparation and Storage
Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze/thaw cycles. When stored properly, products should be stable for at least 5 months.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Other Notes
Small volumes of PHGDH antibody pair vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for PHGDH antibody pair
Sandwich ELISA (EIA)
Application Notes for PHGDH antibody pair
We recommend using the capture antibody at a concentration of 0.5ug/ml and the detection antibody at a concentration of 0.2ug/ml.
NCBI/Uniprot data below describe general gene information for PHGDH. It may not necessarily be applicable to this product.
NCBI Accession #
CAG33076.1
[Other Products]
UniProt Primary Accession #
O43175
[Other Products]
UniProt Secondary Accession #
Q5SZU3; Q9BQ01; B2RD08[Other Products]
UniProt Related Accession #
O43175[Other Products]
Molecular Weight
56,651 Da
NCBI Official Full Name
PHGDH
NCBI Official Synonym Full Names
phosphoglycerate dehydrogenase
NCBI Official Symbol
PHGDH??[Similar Products]
NCBI Official Synonym Symbols
NLS; PDG; PGD; NLS1; PGAD; PGDH; SERA; 3PGDH; 3-PGDH; PHGDHD; HEL-S-113
??[Similar Products]
NCBI Protein Information
D-3-phosphoglycerate dehydrogenase
UniProt Protein Name
D-3-phosphoglycerate dehydrogenase
UniProt Gene Name
PHGDH??[Similar Products]
UniProt Synonym Gene Names
PGDH3; 3-PGDH??[Similar Products]
UniProt Entry Name
SERA_HUMAN
NCBI Summary for PHGDH
This gene encodes the enzyme which is involved in the early steps of L-serine synthesis in animal cells. L-serine is required for D-serine and other amino acid synthesis. The enzyme requires NAD/NADH as a cofactor and forms homotetramers for activity. Mutations in this gene have been found in a family with congenital microcephaly, psychomotor retardation and other symptoms. Multiple alternatively spliced transcript variants have been found, however the full-length nature of most are not known. [provided by RefSeq, Aug 2011]
UniProt Comments for PHGDH
PHGDH: Defects in PHGDH are the cause of phosphoglycerate dehydrogenase deficiency (PHGDH deficiency). It is characterized by congenital microcephaly, psychomotor retardation, and seizures. Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.
Protein type: Cell development/differentiation; Oxidoreductase; Amino Acid Metabolism - glycine, serine and threonine; EC 1.1.1.95
Chromosomal Location of Human Ortholog: 1p12
Cellular Component: cytosol; myelin sheath
Molecular Function: electron carrier activity; NAD binding; phosphoglycerate dehydrogenase activity
Biological Process: brain development; gamma-aminobutyric acid metabolic process; glial cell development; glutamine metabolic process; glycine metabolic process; L-serine biosynthetic process; neural tube development; neurite development; regulation of gene expression; serine family amino acid biosynthetic process; spinal cord development; taurine metabolic process; threonine metabolic process
Disease: Neu-laxova Syndrome 1; Phosphoglycerate Dehydrogenase Deficiency
Research Articles on PHGDH
1. High expression of PHGDH is associated with Colon Cancer.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
