Full Product Name
PHGDH, ID (PHGDH, PGDH3, D-3-phosphoglycerate dehydrogenase)
Product Synonym Names
Anti -PHGDH, ID (PHGDH, PGDH3, D-3-phosphoglycerate dehydrogenase)
Product Gene Name
anti-PHGDH antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 1; NC_000001.10 (120254419..120286849). Location: 1p12
3D Structure
ModBase 3D Structure for O43175
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Immunogen
PHGDH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 256-285 amino acids from the Central region of human PHGDH.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Other Notes
Small volumes of anti-PHGDH antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-PHGDH antibody
3-Phosphoglycerate dehydrogenase (PHGDH; EC 1.1.1.95) catalyzes the transition of 3-phosphoglycerate into 3-phosphohydroxypyruvate, which is the first and rate-limiting step in the phosphorylated pathway of serine biosynthesis, using NAD+/NADH as a cofactor.
Product Categories/Family for anti-PHGDH antibody
Antibodies; Abs to Enzymes, Dehydrogenase
Applications Tested/Suitable for anti-PHGDH antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes for anti-PHGDH antibody
Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100
Flow Cytometry: 1:10-50
NCBI/Uniprot data below describe general gene information for PHGDH. It may not necessarily be applicable to this product.
NCBI Accession #
CAG33076.1
[Other Products]
UniProt Primary Accession #
O43175
[Other Products]
UniProt Secondary Accession #
Q5SZU3; Q9BQ01; B2RD08[Other Products]
UniProt Related Accession #
O43175[Other Products]
Molecular Weight
56,651 Da[Similar Products]
NCBI Official Full Name
PHGDH
NCBI Official Synonym Full Names
phosphoglycerate dehydrogenase
NCBI Official Symbol
PHGDH??[Similar Products]
NCBI Official Synonym Symbols
PDG; PGD; PGAD; PGDH; SERA; 3PGDH; 3-PGDH
??[Similar Products]
NCBI Protein Information
D-3-phosphoglycerate dehydrogenase
UniProt Protein Name
D-3-phosphoglycerate dehydrogenase
UniProt Gene Name
PHGDH??[Similar Products]
UniProt Synonym Gene Names
PGDH3; 3-PGDH??[Similar Products]
UniProt Entry Name
SERA_HUMAN
NCBI Summary for PHGDH
This gene encodes the enzyme which is involved in the early steps of L-serine synthesis in animal cells. L-serine is required for D-serine and other amino acid synthesis. The enzyme requires NAD/NADH as a cofactor and forms homotetramers for activity. Mutations in this gene have been found in a family with congenital microcephaly, psychomotor retardation and other symptoms. Multiple alternatively spliced transcript variants have been found, however the full-length nature of most are not known. [provided by RefSeq, Aug 2011]
UniProt Comments for PHGDH
PHGDH: Defects in PHGDH are the cause of phosphoglycerate dehydrogenase deficiency (PHGDH deficiency). It is characterized by congenital microcephaly, psychomotor retardation, and seizures. Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.
Protein type: Cell development/differentiation; EC 1.1.1.95; Amino Acid Metabolism - glycine, serine and threonine; Oxidoreductase
Chromosomal Location of Human Ortholog: 1p12
Cellular Component: cytosol
Molecular Function: electron carrier activity; phosphoglycerate dehydrogenase activity; NAD binding
Biological Process: glial cell development; neural tube development; glycine metabolic process; L-serine biosynthetic process; regulation of gene expression; spinal cord development; gamma-aminobutyric acid metabolic process; glutamine metabolic process; amino acid biosynthetic process; brain development; taurine metabolic process; neurite development; threonine metabolic process
Disease: Phosphoglycerate Dehydrogenase Deficiency; Neu-laxova Syndrome 1
Research Articles on PHGDH
1. The potential mechanisms by which PHGDH promotes cancer are discussed.
Precautions
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Disclaimer
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