For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

IgG

Rabbit

Affinity purification

Store at -20 degree C. Avoid freeze / thaw cycles.

Small volumes of anti-GRHL2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Polyclonal

Western Blot (WB)

WB: 1:500 - 1:2000

Western blot analysis of extracts of SH-SY5Y cells, using GRHL2 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

Calculated MW: 71kDa
Molecular Weight: 625

grainyhead like transcription factor 2

grainyhead-like protein 2 homolog

Grainyhead-like protein 2 homolog

BOM; TFCP2L3??[Similar Products]

GRHL2_HUMAN

The protein encoded by this gene is a transcription factor that can act as a homodimer or as a heterodimer with either GRHL1 or GRHL3. Defects in this gene are a cause of non-syndromic sensorineural deafness autosomal dominant type 28 (DFNA28).[provided by RefSeq, Mar 2009]

GRHL2: May function as a transcription factor. Defects in GRHL2 are the cause of deafness autosomal dominant type 28 (DFNA28). DFNA28 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. DFNA28 is characterized by mild to moderate hearing loss across most frequencies that progressed to severe loss in the higher frequencies by the fifth decade. Age at onset varied, with the earliest case documented at 7 years of age. Belongs to the grh/CP2 family. Grainyhead subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor

Chromosomal Location of Human Ortholog: 8q22.3

Cellular Component: intercellular junction; nucleoplasm; nucleus; plasma membrane

Molecular Function: chromatin DNA binding; protein binding; sequence-specific DNA binding; transcription factor activity

Biological Process: brain development; camera-type eye development; cell adhesion; cell proliferation; embryonic cranial skeleton morphogenesis; embryonic digit morphogenesis; epidermis development; multicellular organism growth; negative regulation of keratinocyte differentiation; neural tube closure; neural tube development; positive regulation of telomerase activity; positive regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter

Disease: Deafness, Autosomal Dominant 28; Ectodermal Dysplasia/short Stature Syndrome

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