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Elastase 2 (ELA2), ELISA Kit

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產(chǎn)品名稱: Elastase 2 (ELA2), ELISA Kit
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Elastase 2 (ELA2), ELISA Kit


Elastase 2 (ELA2), ELISA Kit  的詳細(xì)介紹
Product Name

Elastase 2 (ELA2), ELISA Kit

Full Product Name

Mouse Elastase 2, Neutrophil ELISA Kit (ELA2)

Product Gene Name

ELA2 elisa kit

[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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Request for Current Manual Insert
Request Current Manual
3D Structure
ModBase 3D Structure for Q3UP87
Species Reactivity
Mouse
Assay Type
Sandwich ELISA
Samples
Cell Culture Supernatant, Serum, Plasma (EDTA, Citrate, Heparin)
Detection Range
0.156-10 ng/mL
Sensitivity
0.062 ng/mL
Detection
Colorimetric
Conjugate
HRP
Preparation and Storage
Store at 4 degree C.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of ELA2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for ELA2 purchase
MBS9138292 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Elastase 2 (ELA2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ELA2. The ELISA analytical biochemical technique of the MBS9138292 kit is based on ELA2 antibody-ELA2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ELA2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ELA2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for
ELA2 elisa kit
Product Overview: The ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of samples in cell culture supernatant, serum, plasma (EDTA, citrate, heparin).
Intended Use: This Mouse Elastase 2, Neutrophil (ELA2) ELISA kit is intended for laboratory research use only and not for use in diagnostic or therapeutic procedures. The stop solution changes color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of Mouse Elastase 2, Neutrophil (ELA2) in the sample, this Mouse Elastase 2, Neutrophil (ELA2) ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of optical density versus Mouse Elastase 2, Neutrophil (ELA2) concentration. The concentration of the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI/Uniprot data below describe general gene information for ELA2. It may not necessarily be applicable to this product.
NCBI GI #
114052444
NCBI GeneID
50701
NCBI Accession #
NP_056594.2 [Other Products]
NCBI GenBank Nucleotide #
NM_015779.2 [Other Products]
UniProt Primary Accession #
Q3UP87 [Other Products]
UniProt Secondary Accession #
Q61515; A6H698[Other Products]
UniProt Related Accession #
Q3UP87[Other Products]
Molecular Weight
28,648 Da
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NCBI Official Full Name
neutrophil elastase
NCBI Official Synonym Full Names
elastase, neutrophil expressed
NCBI Official Symbol
Elane??[Similar Products]
NCBI Official Synonym Symbols
NE; Ela2; F430011M15Rik
??[Similar Products]
NCBI Protein Information
neutrophil elastase
UniProt Protein Name
Neutrophil elastase
UniProt Synonym Protein Names
Elastase-2; Leukocyte elastase
UniProt Gene Name
Elane??[Similar Products]
UniProt Synonym Gene Names
Ela2??[Similar Products]
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NCBI Summary for ELA2
This gene encodes a member of the chymotrypsin-like family of serine protease enzymes that hydrolyzes a broad range of protein substrates including elastin. This gene is expressed by neutrophils where the encoded enzyme is stored in azurophil granules. Upon neutrophil activation, the active enzyme is released into the extracellular mileu. Mice lacking the encoded protein exhibit increased susceptibility to sepsis and death following intraperitoneal infection with Gram negative bacteria. This gene is located adjacent to a related proteinase gene on chromosome 10. [provided by RefSeq, Jul 2016]
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UniProt Comments for ELA2
Medullasin modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis ().
Research Articles on ELA2
1. the impact of NE mutations that are typical of human congenital neutropenia in a murine model of in vitro and in vivo neutrophil differentiation
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Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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